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invalid feature coordinates (end<start!) at line #131

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tky199996 opened this issue Feb 27, 2024 · 1 comment
Open

invalid feature coordinates (end<start!) at line #131

tky199996 opened this issue Feb 27, 2024 · 1 comment

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@tky199996
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Hello, after I converted the bed file to a gtf file, I planned to convert the gtf file to fa, but the following error was displayed. How should I solve it? thank you very much for your help
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@tky199996
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First, I used pbmm2 to align the lfnc sequence to the reference genome sequence and generate a bam file. Then I converted the bam file into a sam file, using the samtools tool for conversion, then using the tama_collapse.py command, and then using tama_merge.py command, but subsequently when I continue to use the SQANTI3 command, it prompts me the following, how can I solve it, does this have any impact?
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