FindConservedMarkers.Rd

% Generated by roxygen2: do not edit by hand
% Please edit documentation in R/differential_expression.R
\name{FindConservedMarkers}
\alias{FindConservedMarkers}
\title{Finds markers that are conserved between the groups}
\usage{
FindConservedMarkers(
  object,
  ident.1,
  ident.2 = NULL,
  grouping.var,
  assay = "RNA",
  slot = "data",
  min.cells.group = 3,
  meta.method = metap::minimump,
  verbose = TRUE,
  ...
)
}
\arguments{
\item{object}{An object}

\item{ident.1}{Identity class to define markers for}

\item{ident.2}{A second identity class for comparison. If NULL (default) -
use all other cells for comparison.}

\item{grouping.var}{grouping variable}

\item{assay}{of assay to fetch data for (default is RNA)}

\item{slot}{Slot to pull data from; note that if \code{test.use} is "negbinom", "poisson", or "DESeq2",
\code{slot} will be set to "counts"}

\item{min.cells.group}{Minimum number of cells in one of the groups}

\item{meta.method}{method for combining p-values. Should be a function from
the metap package (NOTE: pass the function, not a string)}

\item{verbose}{Print a progress bar once expression testing begins}

\item{\dots}{parameters to pass to FindMarkers}
}
\value{
data.frame containing a ranked list of putative conserved markers, and
associated statistics (p-values within each group and a combined p-value
(such as Fishers combined p-value or others from the metap package),
percentage of cells expressing the marker, average differences). Name of group is appended to each
associated output column (e.g. CTRL_p_val). If only one group is tested in the grouping.var, max
and combined p-values are not returned.
}
\description{
Finds markers that are conserved between the groups
}
\examples{
\dontrun{
data("pbmc_small")
pbmc_small
# Create a simulated grouping variable
pbmc_small[['groups']] <- sample(x = c('g1', 'g2'), size = ncol(x = pbmc_small), replace = TRUE)
FindConservedMarkers(pbmc_small, ident.1 = 0, ident.2 = 1, grouping.var = "groups")
}

}
\concept{differential_expression}