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This repository contains two module including residual contextual and subpixel convolution network (RC-SPCNet) and post-processing (lifted multicut), developed by Hainan university and Institute of Automation, Chinese Academy of Sciences.

Environment

The code is developed and tested under the following configurations.

• Network

CUDA>=11.0, Python>=3.7, tensorflow-gpu>=1.14.0, keras>=2.2.5

• Lifted multicut

Python>=3.7, nifty, elf, vigra, numpy, imageio, napari

Installation

We recommend creating new environments independently.

• Download the repository

• Install the required python environment

• Activate your new environment and run the code

For the post-processing method, we suggest install the package via conda and pip as follow:

conda install -c conda-forge vigra

conda install -c conda-forge nifty

conda install -c conda-forge python-elf

python -m pip install imageio

python -m pip install "napari[all]"

Test Data

The dataset of lifted multicut can be download in: https://pan.baidu.com/s/1_fX2FNKDXMMSWe8pQOnh4g, code:ydlo

How to use (lifted multicut)

Adjust the data path and run the code “postprocess_lifted_multicut.py”, the result will be saved in the specified path.

Acknowledgement

This project is built upon some previous projects. Especially, we'd like to thank the contributors of the following github repositories: multicut_pipeline: https://github.com/ilastik/nature_methods_multicut_pipeline and elf: https://github.com/constantinpape/elf

Contact

If you meet any problem, please contact the author directly.

Email: [email protected]