Fast-NNT is both an executable binary that can be run, and a library that can be used in Rust programs.

Installing the `fast_nnt` executable

Assuming you have Rust/Cargo installed, run this command in a terminal:

cargo install fast-nnt

It will make the fast_nnt command available in your PATH if you've allowed the PATH to be modified when installing Rust. cargo uninstall fast-nnt uninstalls.

Adding `fast_nnt` library as a dependency

Run this command in a terminal, in your project's directory:

cargo add fast-nnt

To add it manually, edit your project's Cargo.toml file and add to the [dependencies] section:

fast-nnt = "0.2.4"

The fast_nnt library will be automatically available globally. Read the fast_nnt library documentation.

Back to the crate overview.

Readme

fast-nnt

fast-nnt (read Fast Ent) is a simple Rust implementation of the Neighbour Net algorithm with both R and Python bindings.

Introduction

Fast-NNT is a Rust implementation of the NeighbourNet algorithm, designed for efficient phylogenetic analysis. It constructs split trees from distance matrices, providing a fast and reliable tool for researchers in evolutionary biology. R and Python bindings are provided so that users can easily integrate Fast-NNT into their existing workflows.

Why does this exist when SplitsTree is available?

Well, SplitsTree4/6 are GUI-based applications, while Fast-NNT is a command-line tool that can be easily integrated into automated workflows and pipelines. It's meant to be lightweight and simple to use, you provide a simple input and it generates the nexus file. You can then use this file in R or Python to generate your own plots. This is perfect for people who love to manually beautify their visualizations.

Additionally, we provide R and Python bindings that allow you pass in memory data matrices directly with a single command and get results without having to write intermediate files. For installation instructions, please refer to the respective documentation for R and Python.

How do the results compare to SplitsTree?

Fast-NNT aims to produce results that are consistent with those generated by SplitsTree, but there may be differences due to the underlying implementations and algorithms used. Users are encouraged to compare the output from Fast-NNT with that of SplitsTree to assess any discrepancies and determine the best tool for their specific needs.

Comparison to SplitsTree4

Installation

Install Rust via rustup.

Python

You can install the Python package via pip:

pip install fastnntpy

R

You can install the R package via:

install.packages("fastnntr")

# Or if CRAN is unavailable and you have Rust on your machine, i think this will work
devtools::install_github("rhysnewell/fast-nnt", subdir = "fastnntr")

If you are developing locally and have changed the R bindings:

rextendr::document()
devtools::load_all()

CLI

cargo install fast-nnt

Alternatively, you can build from source. Clone and install this repo via:

git clone https://github.com/rhysnewell/fast-nnt.git
cd fast-nnt
cargo install --path .

Usage

For Python and R, complete usage examples can be found in test/python and test/R. But a brief summary is as follows.

Ordering and inference methods

Fast-NNT exposes two knobs that match the algorithmic choices in SplitsTree:

Ordering (ordering_method / -O): the cycle construction step. Options are splitstree4 (SplitsTree4-style ordering) and huson2023 (the improved ordering from Bryant & Huson 2023). The default is huson2023.
Inference (inference_method / --inference): the split-weight solver. Options are active-set (active-set NNLS, default) and splitstree4 (SplitsTree4-style optimizer).
For SplitsTree6-style defaults, use ordering_method="splitstree4" with inference_method="active-set" (or -O splits-tree4 --inference active-set in the CLI).
For SplitsTree4-style defaults, use ordering_method="splitstree4" with inference_method="splitstree4" (or -O splits-tree4 --inference splitstree4 in the CLI).

Python

Read data in via numpy, pandas, or polars:

import fastnntpy as fn
import pandas as pd
data = pd.read_csv("test/data/large/large_dist_matrix.csv")
n = fn.run_neighbour_net(
    data,
    ordering_method="huson2023",
    inference_method="active-set",
)
print("Labels")
print(len(n.get_labels()))
print("Splits Records")
print(len(n.get_splits_records()))
print("Node Translations")
print(len(n.get_node_translations()))
print("Node Positions")
print(len(n.get_node_positions()))
print("Graph Edges")
print(len(n.get_graph_edges()))

R

Read your distance matrix in using your preferred method (e.g., data.table):

library(fastnntr)
library(data.table)
data <- fread("test/data/large/large_dist_matrix.csv", header=TRUE)
# Load network
Nnet <- fastnntr::run_neighbournet_networkx(
  data,
  ordering_method="huson2023",
  inference_method="active-set"
)

The run_neighbournet_networkx function will return an object almost identical to that produced by phangorn, so should be compatible with existing workflows.

CLI

Required input is a symmetrical distance matrix, ideally with a header row indicating the taxa labels. Can be separated by any delimiter.

To generate a split nexus file (mostly) identical to SplitsTree4 and SplitsTree6:

fast_nnt neighbour_net -t 4 -i test/data/large_dist_matrix.csv -d output_dir -o prefix -O splits-tree4

Use the new Huson 2023 ordering algorithm (default):

fast_nnt neighbour_net -t 4 -i test/data/large_dist_matrix.csv -d output_dir -o prefix -O huson2023

Select the split-weight inference method:

fast_nnt neighbour_net -t 4 -i test/data/large_dist_matrix.csv -d output_dir -o prefix --inference active-set
fast_nnt neighbour_net -t 4 -i test/data/large_dist_matrix.csv -d output_dir -o prefix --inference splitstree4

Output

The output will include a nexus file containing the split network and network layout.

Citations

If you use this tool in your work, please cite the original authors work:

Bryant & Huson 2023: D. Bryant and DH Huson, NeighborNet- improved algorithms and implementation. Front. Bioinform. 3, 2023.
Bryant & Moulton 2004: D. Bryant and V. Moulton. Neighbor-net: An agglomerative method for the construction of phylogenetic networks. Molecular Biology and Evolution, 21(2):255– 265, 2004.

You can also cite this repository directly:

Newell, R. J. P., & McMaster, E. S. (2025). Fast-NNT: Fast NeighbourNet Split Trees For Unrooted Phylogenetic Analysis (v0.2.0). Zenodo. https://doi.org/10.5281/zenodo.16907379

Installing the fast_nnt executable

Adding fast_nnt library as a dependency

Installing the `fast_nnt` executable

Adding `fast_nnt` library as a dependency