BLAST (Basic Local Alignment Search Tool)

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E -value minimum and Score maximum for best allignment

Example for a Gen bank file. Data can be submitted either by offline SEQUIN or online BankIt(data submission tools)

CLUSTALW multiple allignment

Sequences to be aligned are pasted on a note pad Example : following 7 sequences are to be aligned
>gi|109892226|gb|DQ501260.1| Zombitsia lucorum ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene, partial cds; chloroplast TGACTTATTATACTCCTGAATATGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCA ACCGGGAGTTCCACCTGAGGAAGCAGGGGCCGCTGTAGCTGCTGAATCTTCTACTGGTACATGGACAACT GTGTGGACCGATGGGCTTACCAGTCTTGATCGTTACAAAGGACGATGCTATGGAATCGAGCCTGTTCCTG GAGAAGAAAATCAATATATTGCTTATGTAGCTTATCCCCTAGACCTTTTTGAAGAAGGTTCTGTTACTAA CATGTTTACTTCCATTGTGGGTAACGTATTTGGATTCAAGGCTCTACGTGCTCTACGTCTGGAGGATTTG CGAATCCCTACTGCTTATATTAAAACTTTCCAAGGCCCGCCTCATGGTATCCAGGTTGAAAGAGATAAAT TGAACAAGTATGGTCGCCCTCTATTGGGATGTACTATTAAACCAAAATTGGGATTATCCGCTAAGAATTA TGGTAGAGCAGTTTATGAATGTCTACGCGGTGGACTTGATTTTACCAAAGATGATGAAAACGTGAATTCC CAACCATTTATGCGTTGGAGAGACCGTTTCCTATTTTGTGCGGAAGCTATTTTTAAATCACAGGCTGAAA CAGGTGAAATCAAGGGACATTACTTGAATGCTACTGCAGGTACATGCGAAGAAATGATCAAAAGGGCTGT ATTTGCCCGAGAATTGGGAGTTCCTATCGTAATGCATGACTACTTAACAGGTGGATTCACTGCAAATACT AGCTTGGCTCATTATTGCCGAGATAATGGTCTACTTCTTCACATTCACCGTGCAATGCATGCCGTTATTG ATAGACAGAAGAATCATGGTATGCACTTCCGTGTACTAGCTAAAGCCTTACGTATGTCTGGTGGAGACCA TATTCACGCTGGTACCGTAGTAGGTAAACTTGAAGGGGAAAGAGAAATCACTTTAGGCTTTGTTGATTTA CTACGTGATGATTTTATTGAAAAAGACCGAAGCCGCGGTATTTATTTCACTCAAGATTGGGTCTCTTTAC CAGGTGTTCTACCAGTGGCTTCCGGTGGTATTCACGTTTGGCATATGCCTGCTCTAACCGAGATTTTTGG AGATGATTCTGTACTACAATTCGGCGGAGGAACTTTGGGGCACCCTTGGGGTAATGCACCTGGTGCCGTA GCTAACCGAGTAGCTCTAGAAGCATGTGTACAAGCTCGTAATGAGGGACGTGATCTTGCTCGTGAGGGTA ATGAAATTATCCGTGAGGCTAGTAAATGGAGTCCTGAACTAGCTGCTGCTTGTGAAGTATGGAAGGAGAT CAAATTTGAATTTGAGGCAATGGATACTTTGTAATCCAG >gi|109892224|gb|DQ501259.1| Tricyclandra leandrii ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene, partial cds; chloroplast ACTTATTATACTCCTGAATATCAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAAC CGGGAGTTCCACCCGAGGAAGCAGGGGCTGCTGTAGCTGCTGAATCTTCTACTGGTACATGGACAACTGT

> this sign is important

GTGGACCGATGGGCTTACCAGTCTTGATCGTTACAAAGGACGATGCTATAACATCGAGCCTGTTGCTGGA GAAGAAAATCAATATATTGCTTATGTAGCTTATCCCCTAGACCTTTTTGAAGAAGGTTCTGTTACTAACA TGTTTACTTCCATTGTGGGTAATGTATTTGGATTCAAGGCTCTGCGCGCTCTACGTCTGGAGGATTTGCG AATCCCTCCTGCTTATATTAAAACTTTCCAAGGCCCACCTCATGGTATCCAGGTTGAAAGAGATAAATTG AACAAGTATGGCCGCCCTCTATTGGGATGTACTATTAAACCAAAATTGGGATTATCCGCTAAGAATTATG GTAGAGCAGTTTATGAATGTCTACGCGGTGGACTTGATTTTACCAAAGATGATGAAAACGTGAATTCCCA ACCATTTATGCGTTGGAGAGACCGTTTCCTATTTTGTGCGGAAGCCATTTATAAATCACAGGCTGAAACA GGTGAAATCAAGGGACATTACTTGAATGCTACTGCAGGTACATGCGAAGAAATGATCAAAAGGGCTGTAT TTGCGAGAGAATTGGGAGTTCCTATCGTAATGCATGACTACTTAACAGGTGGATTCACTGCAAATACTAG CTTGGCTCATTATTGCCGAGATAATGGTCTACTTCTTCACATTCACCGTGCAATGCATGCCGTTATTGAT AGACAGAAGAATCATGGTATGCACTTCCGTGTACTAGCTAAAGCGTTACGTATGTCTGGTGGAGACCATA TTCACGCTGGTACCGTAGTAGGTAAACTTGAAGGGGAAAGAGAAATCACTTTAGGCTTTGTTGATTTACT ACGTGATGATTTTGTTGAGAAAGATCGAAGCCGCGGTATTTATTTCACTCAAGATTGGGTCTCTTTACCA GGTGTTCTGCCAGTGGCTTCCGGTGGTATTCACGTTTGGCATATGCCTGCTCTGACCGAGATTTTTGGAG ATGATTCTGTACTACAATTCGGCGGAGGAACTTTAGGGCACCCTTGGGGTAATGCACCTGGTGCCGTAGC TAATCGAGTAGCTCTAGAAGCATGTGTAAAAGCTCGTAATGAGGGACGTGATCTTGCTCGTGAGGGTAAT GAAATTATCCGTGAGGCTAGTAAATGGAGTCCTGAACTAGCTGCTGCTTGTGAAGTATGGAA >gi|109892222|gb|DQ501258.1| Baijiania yunnanensis ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene, partial cds; chloroplast GTTGGATTCAAAGCTGGTGTTAAAGATTATAAATTGACTTATTATACTCCTGACTATGAAACCAAAGATA CTGATATCTTGGCAGCATTCCGAGTAACTCCTCAACCGGGAGTTCCACCCGAGGAAGCAGGGGCCGCTGT AGCTGCTGAATCTTCGACTGGTACATGGACAACTGTGTGGACCGATGGGCTTACCAGTCTTGATCGTTAC AAAGGACGATGCTATGACATCGAGCCTGTTCCTGGAGAAGAAAGTCAATTTATTGCTTATGTAGCTTATC CCCTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTGGGTAATGTATTTGGGTT CAAAGCTCTGCGCGCTCTACGTCTGGAGGATTTGCGAATCCCTACTGCTTATATTAAAACTTTCCAAGGC CCGCCTCATGGTATCCAAGTTGAGAGAGATAAATTGAACAAGTATGGCCGCCCTCTATTGGGATGTACTA TTAAACCAAAATTGGGATTATCCGCTAAGAATTATGGTAGAGCAGTTTATGAATGTCTACGCGGTGGACT TGATTTTACCAAAGATGATGAAAACGTGAATTCCCAACCATTTATGCGTTGGAGAGATCGTTTCCTATTT TGTGCGGAAGCCATTTTTAAATCACAGGCTGAAACAGGTGAAATCAAGGGACATTACTTGAATGCTACTG CAGGTACATGCGAAGAAATGATGAAAAGGGCTATATTTGCCAGAGAATTAGGAGTTCCTATCGTAATGCA TGACTACTTAACAGGTGGATTCACTGCAAATACTAGCTTGGCTCATTATTGCCGAGATAATGGTCTACTT CTTCACATTCACCGTGCAATGCATGCCGTTATTGATAGACAGAAGAATCATGGTATGCACTTTCGTGTAC

TAGCTAAAGCGTTACGTCTGTCTGGTGGAGATCATATTCACGCTGGTACCGTAGTAGGTAAACTTGAAGG GGAAAGAGACATCACTTTAGGCTTTGTTGATTTACTACGTGATGATTTTGTTGAAAAAGACCGAAGCCGC GGTATTTATTTCACTCAAGATTGGGTCTCTTTACCTGGTGTTCTGCCAGTGGCTTCCGGGGGTATTCACG TTTGGCATATGCCTGCTCTGACCGAGATTTTTGGAGATGATTCTGTACTACAATTCGGCGGAGGAACTTT AGGGCACCCTTGGGGTAATGCACCTGGTGCCGTAGCTAACCGAGTAGCTCTAGAAGCATGTGTACAAGCT CGTAATGAGGGACGTGATCTTGCTCGTGAGGGTAATGAAATTATCCGTGAGGCTAGTAAATGGAGTCCTG AACTAGCTGCTGCTTGTGAAGTATGGAAGGCGATCAAATTTGAATTTGATGCAGTAGATACTTTGTA >gi|109892220|gb|DQ501257.1| Lemurosicyos variegatus ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene, partial cds; chloroplast AAAGATTATAAATTGACTTATTATACTCCTGAATATGAAACCAAAGATACTGATATCTTGGCAGCATTCC GAGTAACTCCTCAACCGGGAGTTCCACCTGAGGAAGCAGGGGCCGCTGTAGCTGCTGAATCTTCTACTGG TACATGGACAACTGTGTGGACCGATGGGCTTACCAGTCTTGATCGTTACAAAGGACGATGCTATGGAATC GAGCCTGTTCCTGGAGAAGAAAATCAATATATTGCTTATGTAGCTTATCCCCTAGACCTTTTTGAAGAAG GTTCTGTTACTAACATGTTTACTTCCATTGTGGGTAATGTATTTGGATTCAAGGCTCTACGTGCTCTACG TCTGGAGGATTTGCGAATCCCTATTGCTTATATTAAAACTTTCCAAGGCCCGCCTCATGGTATCCAGGTT GAAAGAGATAAATTGAACAAGTATGGTCGCCCTCTATTGGGATGTACTATTAAACCAAAATTGGGATTAT CCGCTAAGAATTATGGTAGAGCAGTTTATGAATGTCTACGCGGTGGACTTGATTTTACCAAAGATGATGA AAACGTGAATTCCCAACCATTTATGCGTTGGAGAGACCGTTTCCTATTTTGTGCGGAAGCTATTTTTAAA TCACAGGCTGAAACAGGTGAAATCAAGGGACATTACTTGAATGCTACTGCAGGTACATGCGAAGAAATGA TGAAAAGGGCTGTATTTGCTCGAGAATTGGGAGTTCCTATCGTAATGCATGACTACTTAACCGGTGGATT CACTGCAAATACTAGCTTGGCTCATTATTGCCGAGATAATGGTCTACTTCTTCACATTCACCGTGCAATG CATGCCGTTATTGATAGACAGAAGAATCATGGTATGCACTTCCGTGTACTAGCTAAAGCGTTACGTATGT CTGGTGGAGACCATATTCACGCTGGTACCGTAGTAGGTAAACTTGAAGGGGAAAGAGAAATCACTTTAGG CTTTGTTGATTTACTACGTGATGATTTTATTGAAAAAGACCGAAGCCGCGGTATTTATTTCACTCAAGAT TGGGTCTCTTTACCAGGTGTTCTGCCAGTGGCTTCCGGTGGTATTCACGTTTGGCATATGCCTGCTCTAA CCGAGATTTTTGGAGATGATTCTGTACTACAATTCGGCGGAGGAACTTTGGGGCACCCTTGGGGTAATGC ACCTGGTGCCGTAGCTAACCGAGTAGCTCTAGAAGCATGTGTACAAGCTCGTAATGAGGGACGTGATCTT GCTCGTGAGGGTAATGAAATTATCCGTGCGGCTAGTAAATGGAGTCCTGAACTAGCTGCTGCTTGTGAAG TATGGAAGGAGATCAAATTTGAATTTGAGGCAATGGATACTTTGTAATCCAGTAATTACTCTTCGTTCTT TAATGATTCAA

>gi|109892218|gb|DQ501256.1| Indofevillea khasiana ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene, partial cds; chloroplast

ACTTATTATACTCCTGACTATGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAAC CGGGAGTTCCACCCGAGGAAGCAGGGGCCGCTGTAGCTGCTGAATCTTCTACTGGTACATGGACAACTGT GTGGACCGATGGGCTTACCAGTCTTGATCGTTACAAAGGACGATGCTATGACATCGAGCCTGTTCCTGGA GAAGAAAGTCAATTTATTGCTTATGTAGCTTATCCCTTAGACCTTTTTGAAGAAGGTTCTGTTACTAACA TGTTTACTTCCATTGTGGGTAATGTATTTGGGTTCAAGGCTCTGCGTGCTCTACGTCTGGAGGATTTGCG AATCCCTACTGCTTATACTAAAACTTTCCAAGGCCCGCCTCATGGTATCCAAGTTGAGAGAGATAAATTG AACAAGTACGGCCGCCCTCTATTGGGATGTACTATTAAACCAAAATTGGGATTATCCGCTAAGAATTATG GTAGAGCAGTTTATGAATGTCTACGCGGTGGACTTGATTTTACCAAAGATGATGAAAACGTGAATTCCCA ACCATTTATGCGTTGGAGAGACCGTTTCCTATTTTGTGCGGAAGCCATTTATAAATCACAGGCTGAAACA GGTGAAATCAAGGGACATTACTTGAATGCTACTGCAGGTACATGCGAAGAAATGATGAAAAGGGCTGTAT TTGCCAGAGAATTGGGAGTTCCTATCGTAATGCATGACTACTTAACAGGTGGATTCACTGCAAATACTAG CTTGGCTCATTATTGCCGAGATAATGGTCTACTTCTTCACATTCACCGTGCAATGCATGCCGTTATTGAT AGACAGAAGAATCATGGTATGCACTTTCGTGTACTAGCTAAAGCGTTACGTCTGTCTGGTGGAGATCATA TTCACGCTGGTACCGTAGTAGGTAAACTTGAAGGGGAAAGAGACATCACTTTAGGCTTTGTTGATTTACT ACGTGATGATTTTGTTGAAAAAGACCGAAGCCGCGGTATTTATTTCACTCAAGATTGGGTCTCTTTACCA GGTGTTCTGCCAGTGGCTTCCGGGGGTATTCACGTTTGGCATATGCCCGCTCTGACCGAGATTTTTGGAG ATGATTCTGTACTACAATTCGGCGGAGGAACTTTAGGGCATCCTTGGGGTAATGCACCTGGTGCCGTAGC TAATCGAGTAGCTCTAGAAGCATGTGTACAAGCTCGTAATGAGGGACGTGATCTTGCTCGTGAGGGTAAT GAAATTATCCGTGAGGCTAGTAAATGGAGTCCTGAACTAGCTGCTGCTTGTGAAGTATGGAAGGAGATCA AATTTGAATTTG

>gi|109892216|gb|DQ501255.1| Bolbostemma paniculatum ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene, partial cds; chloroplast ACTTATTATACTCCTGAATATGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAAC CCGGAGTTCCACCCGAGGAAGCGGGGGCCGCTGTAGCTGCTGAATCTTCTACTGGTACATGGACAACTGT GTGGACCGATGGGCTTACCAGTCTTGATCGTTACAAAGGACGATGCTACGACATTGAGCCTGTTGCTGGA GAAGAAAATCAATTTATTGCTTATGTAGCTTATCCCCTAGACCTTTTTGAAGAAGGTTCTGTTACTAACA TGTTTACTTCCATTGTGGGTAATGTATTTGGGTTCAAGGCCCTGCGCGCTCTACGTCTGGAGGATTTGCG AATCCCTCCTGCTTATACTAAAACTTTCCAAGGCCCGCCTCATGGTATCCAAGTTGAGAGAGATAAATTG AACAAGTATGGCCGCCCCCTATTGGGATGTACTATTAAACCAAAATTGGGATTATCCGCTAAGAATTATG GTAGAGCTGTTTATGAATGTCTACGCGGTGGACTTGATTTTACCAAAGATGATGAAAACGTGAATTCCCA ACCATTTATGCGTTGGAGAGACCGTTTCCTATTTTGTGCGGAAGCCATTTATAAATCACAGGCTGAAACA

GGTGAAATCAAGGGACATTATTTGAATGCTACTGCGGGTACATGCGAAGAAATGATCAAAAGGGCTGTAT TTGCCAGAGAATTGGGAGTTCCTATCGTAATGCATGACTACTTAACAGGTGGATTCACTGCAAATACTAG CTTGGCTCATTATTGCCGAGATAATGGTCTACTTCTTCACATTCACCGTGCAATGCATGCCGTTATTGAT AGACAGAAGAATCACGGTATGCACTTCCGTGTACTAGCTAAAGCGTTACGTCTGTCTGGTGGAGATCATA TTCACGCTGGTACCGTAGTAGGTAAACTTGAAGGGGAAAGAGATATCACTTTAGGCTTTGTTGATTTACT ACGTGATGATTTTGTTGAAAAAGATCGAAGCCGCGGTATTTATTTCACTCAAGATTGGGTCTCGTTACCT GGTGTTCTGCCGGTGGCTTCCGGGGGTATTCACGTTTGGCATATGCCCGCTCTGACCGAGATTTTTGGAG ATGATTCTGTACTACAATTCGGCGGAGGAACTTTAGGGCACCCTTGGGGAAATGCACCAGGTGCTGTAGC TAATCGAGTAGCTCTAGAAGCATGTGTACAAGCTCGTAATGAGGGACGTGATCTTGCTCGTGAGGGTAAT GAAATTATCCGTGAGGCTTGTAAATGGAGTCCTGAACTAGCTGCTGCTTGTGAAGTATGGAAAGAGATCA AATTTGAATTTGAGGCAATGGATACTTTGTAATCCAGTAATTACT >gi|109892214|gb|DQ501254.1| Ampelosycios humblotii ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene, partial cds; chloroplast ACTTATTATACTCCTGAATATCAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAAC CGGGAGTTCCACCCGAGGAAGCAGGGGCAGCTGTAGCTGCTGAATCTTCTACTGGTACATGGACAACTGT GTGGACCGATGGGCTTACCAGTCTTGATCGTTACAAAGGACGATGCTATGGCATCGAGCCTGTTGCTGGA GAAGAAAATCAATATATTGCTTATGTAGCTTATCCCCTAGACCTTTTTGAAGAAGGTTCTGTTACTAACA TGTTTACTTCCATTGTGGGTAATGTATTTGGATTCAAGGCTCTGCGCGCTCTACGTCTGGAGGATTTGCG AATCCCTCCTGCTTATATTAAAACTTTCCAAGGCCCACCTCATGGTATCCAGGTTGAAAGAGATAAATTG AACAAGTATGGCCGCCCTCTATTGGGATGTACTATTAAACCAAAATTGGGATTATCCGCTAAGAATTATG GTAGAGCAGTTTATGAATGTCTACGCGGTGGACTTGATTTTACCAAAGATGATGAAAACGTGAATTCCCA ACCATTTATGCGTTGGAGAGACCGTTTCCTATTTTGTGCAGAAGCCATTTATAAATCACAGGCTGAAACA GGTGAAATCAAGGGACATTACTTGAATGCTACTGCAGGTACATGCGAAGAAATGATCAAAAGGGCTGTAT TTGCGAGAGAATTGGGAGTTCCTATCGTAATGCATGACTACTTAACAGGTGGATTCACTGCAAATACTAG CTTGGCTCATTATTGCCGAGATAATGGTCTACTTCTTCACATTCACCGTGCAATGCATGCCGTTATTGAT AGACAGAAGAATCATGGTATGCACTTCCGTGTACTAGCTAAAGCGTTACGTATGTCTGGTGGAGACCATA TTCACGCTGGTACCGTAGTAGGTAAACTTGAAGGGGAAAGAGAAATCACTTTAGGCTTTGTTGATTTACT ACGTGATGATTTTGTTGAGAAAGATCGAAGCCGCGGTATTTATTTCACTCAAGATTGGGTCTCTTTACCA GGTGTTCTGCCAGTGGCTTCCGGTGGTATTCACGTTTGGCATATGCCTGCTCTGACCGAGATTTTTGGAG ATGATTCTGTACTCCAATTCGGCGGAGGAACTTTAGGGCACCCTTGGGGTAATGCACCTGGTGCCGTAGC TAATCGAGTAGCTCTAGAAGCATGTGTAAAAGCTCGTAATGAGGGACGTGATCTTGCTCGTGAGGGTAAT GAAATTATCCGTGAGGCTAGTAAATGGAGTCCTGAACTAGCTGCTGCTTGTGAAATATGGAAGGAGATCAAATTTG AATTTGAGGCAATGGATACTTTG

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Analyse option select nucleotide, Non protein coding phylogeny boot strapping 1000 times neighbor joining

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