DNA Replication Scramble
Here are some notes summarising all you need to know about DNA replication. Unfortunately, they
are in the wrong order
nucleotides in a 5 3 direction (In the .
is needed for building up a complementary strand for this template.
To this primer, DN polymerase III adds nucleotides in a 5 3 direction, mo!ing
sealed up by DN ligase "hich ma#es a sugar$phosphate bond bet"een
ad%acent DN fragments.
The DN double heli& is uncoiled and the t"o strands are
DN polymerase III can follo" along behind it, adding nucleotides in one
continuous strand., ho"e!er
a short length of 'N to the template strand of DN, "hich acts as a prime.
a"ay from the replication for# as it does so. In this "ay
replication for# "ill be opening up in the 3 5 direction( another method,
therefore,
short lengths of DN ) called Okaaki fragments $ are formed bet"een 'N
primers.
separated by the en*yme Helicase, producing a replication fork.
reproduce or +replicate a double heli& "ith anti$parallel strands.,
the 'N primer and replaces it "ith DN. nic# is left "here
because the template strands are anti$parallel, for the other template strand,
the
t regular inter!als along the 3 5 strand, 'N primase adds
-ehind the replication for#, the en*yme DN polymerase III adds
Ne&t, DN polymerase I remo!es
t"o nucleotides are still left unconnected ) this nic# is
opposite direction to the direction of the bases in the template strand, so as to
s DN helicase mo!es along one of the anti$parallel template strands in a 5 3
direction
NMA 2011
ANSWER KEY
(., nucleotides in a 5 3 direction (In the .
(/, is needed for building up a complementary strand for this template.
(03, To this primer, DN polymerase III adds nucleotides in a 5 3 direction,
mo!ing
(01, sealed up by DN ligase "hich ma#es a sugar$phosphate bond bet"een
ad%acent DN fragments.
(0, The DN double heli& is uncoiled and the t"o strands are
(3, DN polymerase III can follo" along behind it, adding nucleotides in one
continuous strand., ho"e!er
(02, a short length of 'N to the template strand of DN, "hich acts as a prime.
(03, a"ay from the replication for# as it does so. In this "ay
(5, replication for# "ill be opening up in the 3 5 direction( another method,
therefore,
(05, short lengths of DN ) called Okaaki fragments $ are formed bet"een 'N
primers.
(2, separated by the en*yme Helicase, producing a replication fork.
(04, reproduce or +replicate a double heli& "ith anti$parallel strands.,
(05, the 'N primer and replaces it "ith DN. nic# is left "here
(3,because the template strands are anti$parallel, for the other template strand,
the
(00, t regular inter!als along the 3 5 strand, 'N primase adds
(5, -ehind the replication for#, the en*yme DN polymerase III adds
(0/, Ne&t, DN polymerase I remo!es
(0., t"o nucleotides are still left unconnected ) this nic# is
(1, opposite direction to the direction of the bases in the template strand, so as
to
(3, s DN helicase mo!es along one of the anti$parallel template strands in a 5
3 direction
NMA 2011
