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Bradford Method Protein Assay Guide

The document describes using the Bradford method to determine protein concentrations in samples. Various concentrations of bovine serum albumin (BSA) were used to generate a standard calibration curve. Absorbance readings were taken of the standards and unknown sample when mixed with Bradford reagent to calculate the protein concentration in the unknown based on the calibration curve.

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71 views1 page

Bradford Method Protein Assay Guide

The document describes using the Bradford method to determine protein concentrations in samples. Various concentrations of bovine serum albumin (BSA) were used to generate a standard calibration curve. Absorbance readings were taken of the standards and unknown sample when mixed with Bradford reagent to calculate the protein concentration in the unknown based on the calibration curve.

Uploaded by

ana
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

Protein determination using Bradford method

Samples Samples for the calibration curve Unknown sample Blank


BSA 1 BSA 2 BSA 3 BSA 4 BSA 5
Reagents, μl 0,2 mg/ml 0,4 mg/ml 0,6 mg/ml 0,8 mg/ml 1,0 mg/ml
BSA 0,2 mg/ml 50 - - - - - -
BSA 0,4 mg/ml - 50 - - - - -
BSA 0,6 mg/ml - - 50 - - - -
BSA 0,8 mg/ml - - - 50 - - -
BSA 1,0 mg/ml - - - - 50 - -
Unknown sample - - - - - 50 -
H2O - - - - - - 50
Bradford reagent 1500 1500 1500 1500 1500 1500 1500

Absorbance values 0.092 0.211 0.455 0.561 0.791 0.497 -


(λmax=595 nm)

0.9
0.8
0.7
f(x) = 0.73 x
0.6
Absorbanta

0.5
0.4
0.3
0.2
0.1
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1

Concentratie, mg/ml

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