Course Code BS 153E L T P Total
Course title Downstream Processes in Biotechnology
Scheme and Credits 3 L: 1 T: 0 P 4 Credits
Pre-requisites 1 Basics of biochemical engineering and thermodynamics
2 Principles of chemical engineering or equivalent
Objectives of the 1 Develop expertise in designing and optimizing
course downstream processing equipment.
2 Apply knowledge of chemical, physical, and biochemical
aspects to solve engineering challenges in
bioprocessing
Course title Detailed contents
Downstream 1 Introduction to Downstream Processing, Definition and 5
Processes in importance of downstream processing, Overview of bio
Biotechnology products and their sources, Regulatory considerations
and quality assurance in downstream processing
2 Selection of Separation Processes, Factors influencing 5
the selection of separation techniques, Comparative
analysis of chemical, physical, and biochemical
methods, Case studies: application of different
separation processes in biotechnology
3 Product Release from Cells, Mechanisms of product 5
release from microbial and mammalian cells, Cell
disruption techniques: mechanical, chemical,
enzymatic, Optimization of cell lysis for maximum
product recovery
4 Concentration and Separation Methods, Membrane- 5
based separation processes: ultrafiltration,
microfiltration, reverse osmosis, Ion exchange
chromatography: principles and applications in
biomolecule purification, Laboratory exercises:
membrane filtration and ion exchange chromatography
5 Precipitation and Extraction, Principles of precipitation 5
and solubility in biomolecule purification, Techniques
for selective precipitation of proteins, nucleic acids, and
other biomolecules, Liquid-liquid extraction methods:
solvent extraction, partitioning, extraction kinetics
6 Chromatographic Methods of Purification, Principles of 5
chromatography: adsorption, partition, size exclusion,
affinity, Design and optimization of chromatographic
purification processes, Case studies: application of
chromatography in biopharmaceutical and industrial
biotechnology
7 Design of Downstream Processing Equipment, 5
Equipment selection and design considerations for
downstream bioprocessing, Scale-up and scale-down
strategies for downstream processing operations,
Process integration and automation in downstream
processing facilities
8 Downstream Process Economics, Cost analysis and 5
economic evaluation of downstream processing
operations, Factors influencing process economics: raw
material costs, labour, energy consumption, etc., Case
studies and group projects: economic optimization of
downstream processes
Total 40
Suggested books 1 Belter P.A, Cussler E and Wei Shan Hu, Bioseparation –
Downstream processing for Biotechnology, Wiley
Interscience, 1988.
2 Asenjo and Juan A. Asenjo, Separation Processes in
Biotechnology, CRC Press, 1990.
3 Sivasankar, B. “Bioseparations: Principles and
Techniques”. PHI, 2005
4 Wankat P.C, Rate Controlled Separation, Kluwer
Publishers, 1990.
5 Wang D.I.C, Cooney C.L, Demain A.L, Dunnil.P,
Humphery A.E. and Lilly M.D. Fermentation and Enzyme
Technology, John Wiley and Sons, 1979.
6 Industrial Microbiology & Biotechnology by Arnold L.
demain & Julian E. Davis. (2004) ASM Press.
Outcomes Upon success completion of this course, the students
will be able to:
CO1 Apply principles of various unit operations used in
downstream processing and enhance problem solving
techniques
CO2 Apply ethical and regulatory considerations to
downstream processing activities, ensuring compliance
with industry standards and guidelines.
CO3 Evaluate the economic feasibility of downstream
processing operations and make informed decisions
regarding process optimization.
