Cytology: The Study of Cells
1. Introduction to Cytology
Cytology is the branch of biology that studies the structure, function, and chemistry of cells.
Historical background:
- Robert Hooke (1665): First observed cells in cork.
- Anton van Leeuwenhoek: Observed living cells (bacteria, protozoa).
- Cell Theory: Developed by Schleiden, Schwann, and Virchow.
2. Cell Types
Prokaryotic Cells:
- No nucleus, DNA floats in cytoplasm
- Simple structure (e.g., bacteria)
Eukaryotic Cells:
- True nucleus enclosed in membrane
- Complex structure with organelles (e.g., plant and animal cells)
Diagram: Prokaryotic vs Eukaryotic Cell
3. Cell Structure and Organelles
Organelle Function
Cell membrane Semi-permeable barrier, controls transport
Nucleus Contains DNA, controls activities
Cytoplasm Gel-like fluid, site for reactions
Mitochondria Powerhouse, produces ATP
Endoplasmic Reticulum (RER/SER) Protein (RER) and lipid (SER) synthesis
Golgi apparatus Packaging and transport
Lysosomes Digestion and waste removal
Ribosomes Protein synthesis
Centrioles Cell division (animal cells)
Chloroplasts Photosynthesis (plants)
Cell wall Structure (plants, fungi, bacteria)
Vacuole Storage of substances
Diagram: Labeled Diagram of Animal and Plant Cell
4. Cell Membrane Structure
Fluid Mosaic Model:
- Composed of:
- Phospholipid bilayer
- Proteins (transport, receptor)
- Carbohydrates (signaling)
Diagram: Fluid Mosaic Model with Labels
5. Cell Division
Mitosis: Produces identical cells (growth, repair)
Meiosis: Produces gametes (reduction division)
Diagram: Stages of Mitosis and Meiosis
6. Microscopy in Cytology
Light Microscope: Live cells, limited resolution
Electron Microscope: High resolution (TEM, SEM)
Diagram: Light vs Electron Microscope Comparison
7. Techniques in Cytology
- Cell culture: Growing cells in lab
- Staining: Highlights structures (e.g., iodine, methylene blue)
- Fluorescence microscopy: Uses dyes to view specific organelles
- Flow cytometry: Analyzes physical/chemical properties
8. Applications of Cytology
- Medical diagnostics: e.g., Pap smear, cancer detection
- Research: Gene expression, drug testing
- Forensics: DNA from cells
- Agriculture: GMO development
9. Conclusion
Cytology forms the foundation of biology and medicine by helping us understand the
fundamental unit of life—the cell. Through technological advancements, cytology continues
to open new frontiers in health, genetics, and biotechnology.