223-60668D

Dec.2024

Shimadzu High Performance Liquid Chromatography

LabSolutions GPC

Instruction Manual
Read this manual thoroughly before you use the product.
Keep this manual for future reference.
This page is intentionally left blank.
Introduction Read this Instruction Manual thoroughly
before using the product.
Thank you for purchasing this product.

LabSolutions GPC works as an extension of LabSolutions, and enables you to conduct

GPC analysis on data acquired by LabSolutions under various conditions.

This manual describes the operation, usage cautions for this product. Read this
manual thoroughly before using the product and operate the product in accordance
with the instructions in this manual.

Keep this manual for future reference.

This manual assumes that readers are knowledgeable of basic operation of the LC
workstation software, LabSolutions. Refer to 'Operators Guide' of LabSolutions for
points that are not explained in this manual.

This manual includes contents that are common across the LabSolutions series. Note
that the screen captures inserted in the text may be those of the similar product if there
are no significant discrepancies in the explanation.

Important • If the user or usage location changes, ensure that this manual is always kept
together with the product.

• If this manual is lost or damaged, immediately contact your Shimadzu

representative to request a replacement.

• To ensure safe operation, contact your Shimadzu representative if product

installation, adjustment, or reinstallation (after the product is moved), or repair is
required.

LabSolutions GPC Instruction Manual i

Notice • Information in this manual is subject to change without notice and does not
represent a commitment on the part of the vendor.

• Any errors or omissions which may have occurred in this manual despite the
utmost care taken in its production will be corrected as soon as possible,
although not necessarily immediately after detection.

• All rights are reserved, including those to reproduce this manual or parts thereof
in any form without permission from Shimadzu Corporation.

• Microsoft and Windows are registered trademarks of Microsoft Corporation in the

United States and/or other countries.
Adobe is trademark or registered trademarks of Adobe in the United States and/
or other countries.
Third-party trademarks and trade names may be used in this publication to refer
to either the entities or their products/services, whether or not they are used with
trademark symbol "TM" or "®".

• Screenshots of Microsoft products are used in accordance with guidelines from

Microsoft Corporation.

• Replacement parts for this product will be available for a period of seven (7)
years after the product is discontinued. Thereafter, such parts may cease to be
available.
Note, however, that the availability of parts not manufactured by Shimadzu shall
be determined by the relevant manufacturers.

© 2014-2024 Shimadzu Corporation. All rights reserved.

Original version is approved in English.

ii LabSolutions GPC Instruction Manual

Instruction Manuals
 List of Instruction Manuals

Name Content

Notice Before Using Explains the information necessary to use the LabSolutions GPC, such as how to update, how
to convert the data and caution about the specification of GPC.

Instruction Manual (This Explains data acquisition and analysis procedures of LabSolutions GPC for various purposes.
document)

Quick Manual Explains basic analysis excerpted from the Instruction Manual.

Help Clicking the on-screen [Help] button or pressing the [F1] key displays a description of on-screen
parameters, answers to specific questions or solutions to various problems.
Also, clicking the [Help] button on the error message window displays the details of the error or
solutions to the error. Be sure to refer to Help before contacting us.
For information on how to use it, refer to "8.1.1 Using Help" on page 119 in this manual.

NOTE
Also, refer to the manuals for LabSolutions if necessary. For details, refer to the manuals for LabSolutions.

Reference
"8 Appendix"

 Indications Used in This Manual

The following symbols are used in this manual:

Indication Meaning

NOTE Emphasizes additional information that is provided to ensure the proper use of this product.

Reference Indicates the location of related reference information.

' ' The names of the instruction manuals.

" " The names of programs and products.

[] Indicates the names of buttons, menu options, setting options, windows/sub-windows, and icons
that are displayed in a window.
Example: Click [OK].

[]-[] When several operations are performed one after another, hyphens are used to show the
sequence.
Example: Select [File] - [Print].
Click the [File] menu and select [Print].

LabSolutions GPC Instruction Manual iii

 This page is intentionally left blank.

iv LabSolutions GPC Instruction Manual

 Contents

1 Overview of LabSolutions GPC

1.1 What is GPC? ......................................................................................................1

1.2 Functions of LabSolutions GPC...........................................................................1

1.3 Description of Terms............................................................................................2

2 Starting the Analysis

2.1 Before Reading This Chapter ..............................................................................3
2.1.1 The Data Structure of LabSolutions GPC .......................................................... 3
2.1.2 LabSolutions GPC Operation Flow .................................................................... 3

2.2 Starting LabSolutions Analysis ............................................................................5

2.3 Preparing for Analysis..........................................................................................6

2.3.1 Setting Data Analysis Parameters...................................................................... 6
2.3.2 Saving Method Files......................................................................................... 10

2.4 Analyzing Standard Samples.............................................................................10

2.5 Creating Calibration Curve ................................................................................10

2.5.1 Displaying the [GPC Calibration Curve] Window ............................................. 11
2.5.2 Opening Method File ........................................................................................ 13
2.5.3 Displaying Data Files........................................................................................ 13
2.5.4 Picking up Retention Time ............................................................................... 15
2.5.5 Setting Calibration Curve Parameters.............................................................. 17
2.5.6 Saving Calibration Curves................................................................................ 18

2.6 Creating Report Format .....................................................................................19

2.7 Analyzing Unknown Samples ............................................................................19

2.7.1 Performing Batch Analysis ............................................................................... 19

2.8 Performing GPC Calculation..............................................................................21

2.8.1 Displaying the GPC Data Analysis Results ...................................................... 21
2.8.2 Setting the Parameters for Molecular Weight Calculation................................ 23
2.8.3 Performing GPC Calculation ............................................................................ 25
2.8.4 Viewing Calculation Results ............................................................................. 25

2.9 Comparing GPC Data........................................................................................26

2.9.1 Displaying the [GPC Data Comparison] Window ............................................. 26

LabSolutions GPC Instruction Manual v

Contents

3 Generating Calibration Curves

3.1 Displaying the [GPC Calibration Curve] Window............................................... 29

3.2 Using and Viewing the [GPC Calibration Curve] Window ................................. 30

3.3 Calibration Table View....................................................................................... 31

3.3.1 Calibration Table .............................................................................................. 31
3.3.2 Importing Calibration Points from an ASCII File............................................... 35
3.3.3 Exporting Calibration Table into an ASCII File................................................. 36
3.3.4 Picking Up Peak Data from Chromatogram ..................................................... 37
3.3.5 Calculating Calibration Curve........................................................................... 37
3.3.6 Creating a Calibration Point from Area% ......................................................... 37
3.3.7 Creating a Calibration Point from two known molecular weights ..................... 39

3.4 Calibration Curve View ...................................................................................... 41

3.4.1 Calibration Curve Graph .................................................................................. 41

3.5 Chromatogram View.......................................................................................... 43

3.5.1 Data File List .................................................................................................... 43
3.5.2 [Single] Tab ...................................................................................................... 45
3.5.3 [Multi] Tab ........................................................................................................ 46
3.5.4 [Sample Info.] Tab............................................................................................ 46

3.6 Peak table ......................................................................................................... 47

3.7 Data analysis parameter.................................................................................... 48

4 GPC Calculation
4.1 Displaying the [GPC Data Analysis] Window .................................................... 53

4.2 Using and Viewing the [GPC Data Analysis] Window ....................................... 54

4.3 Chromatogram View.......................................................................................... 55

4.3.1 Display Settings of Chromatograms................................................................. 55
4.3.2 Setting View Properties.................................................................................... 58

4.4 Molecular Weight Distribution View ................................................................... 60

4.4.1 Display Settings of the Molecular Weight Distribution Curves ......................... 60
4.4.2 Setting Properties of Molecular Weight Distribution Curve .............................. 61
4.4.3 Setting Data Analysis Parameters ................................................................... 62
4.4.4 Displaying Slice Data ....................................................................................... 67
4.4.5 Displaying the Results from the Peak Table .................................................... 69
4.4.6 Displaying the Result of the Average Molecular Weight .................................. 70
4.4.7 Displaying the Result of the Average Molecular Weight of the RT/MW Ranges..
71

4.5 GPC Calculation ................................................................................................ 72

4.5.1 Creating a Calibration Curve Table................................................................................................ 72

vi LabSolutions GPC Instruction Manual

 Contents

4.5.2 Approximate Equations for Calibration Curves ................................................ 73

4.5.3 Equations for Calculating Average Molecular Weight ...................................... 73
4.5.4 Time Correction................................................................................................ 74
4.5.5 Time Correction Using the Internal Standard Method ...................................... 74
4.5.6 Time Correction Using the Control Sample...................................................... 74
4.5.7 Sensitivity Compensation of RI Detectors ........................................................ 75

5 GPC Data Comparison

5.1 Displaying the [GPC Data Comparison] Window...............................................77

5.2 Using and Viewing the [GPC Data Comparison] Window .................................78

5.3 Data List View....................................................................................................79

5.4 Chromatogram View ..........................................................................................79

5.4.1 Display of Marker Lines.................................................................................... 80
5.4.2 Shifting Chromatograms................................................................................... 80
5.4.3 Display Settings of Chromatograms................................................................. 81
5.4.4 Setting View Properties .................................................................................... 82

5.5 Molecular Weight Distribution View ...................................................................84

5.5.1 Display of Marker Lines.................................................................................... 84
5.5.2 Display Settings of the Differential Molecular Weight Distribution Curve ......... 84
5.5.3 Setting View Properties .................................................................................... 85

5.6 Cumulative Percent View...................................................................................86

5.6.1 Display of Marker Lines.................................................................................... 86
5.6.2 Setting View Properties .................................................................................... 87

5.7 Statistical Calculation Result .............................................................................88

5.7.1 Setting the Display Items of the [Statistical Calculation Result] Screen........... 89

6 Report Function
6.1 Creating Reports................................................................................................92

6.2 GPC Calibration Curve Item ..............................................................................95

6.3 GPC Graph Item ..............................................................................................101

6.4 GPC Overlay Item............................................................................................102

6.5 GPC Summary Item.........................................................................................103

6.6 Method Item.....................................................................................................108

6.7 GPC Calculation Result ...................................................................................110

7 GPC File Converter

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Contents

7.1 Importing a Method File................................................................................... 115

7.2 Merging GPC Method Files ............................................................................. 116

8 Appendix
8.1 When Having Problems with the Operation..................................................... 119
8.1.1 Using Help...................................................................................................... 119
8.1.2 Using Online Manuals .................................................................................... 120

8.2 Specifications of LabSolutions GPC................................................................ 121

viii LabSolutions GPC Instruction Manual

1 Overview of
LabSolutions GPC
LabSolutions GPC is software that runs on LabSolutions platform. Data acquisition and analysis can
be performed using operations similar to LabSolutions.
This document describes only those items different from LabSolutions. See 'Operation Manual' of
1
LabSolutions for descriptions on "data acquisition", "peak integration" and "batch processing".

1.1 What is GPC?

GPC is short for Gel Permeation Chromatography, and is also called Size Exclusion.
Chromatography. This is a type of separation method used in liquid chromatographs, in which sample
compounds are physically separated by the size of molecules, regardless of any chemical interaction
between sample components and the stationary phase.

1.2 Functions of LabSolutions GPC

LabSolutions GPC is optional software for LabSolutions that calculates molecular weight distribution
and various average molecular weight values from the data acquired by a gel permeation
chromatograph. It enables you to generate parameters and graphs for evaluating characteristics of a
polymer sample using the similar operations with LabSolutions. Furthermore, statistical calculation of
those values and their comparison by overlaying graphs are possible.

 Configuration of the LabSolutions GPC Software

LabSolutions GPC consists mainly of the following three applications:
• GPC Data Analysis
Calculates the molecular weight distribution and average molecular weight values of an unknown sample
from a calibration curve generated by "GPC Calibration Curve".
• GPC Calibration Curve
Generates a calibration curve* that is used to calculate the molecular weight and molecular weight
distribution of an unknown sample.
* A graph showing the relationship between the logarithm of the molecular weight of the samples and the
elution time (eluent volume)
• GPC Data Comparison
Enables you to compare chromatograms and differential and integral molecular weight curves of multiple
unknown samples by overlaying the graphs.
• Batch Postrun
GPC data analysis can be performed for data from multiple samples.
• Report Creation
In addition to the usual report items, report items for outputting GPC analysis results, including molecular
weight distribution calculation results, are added.

 File Compatibility
• GPC method files of CLASS-LC10/CLASS-VP can be imported and used.
• LCsolution GPC data files and method files can be read as they are. However, files created with
LabSolutions GPC cannot be read by LCsolution GPC.

LabSolutions GPC Instruction Manual 1

1 Overview of LabSolutions GPC

1.3 Description of Terms

Following are descriptions on terms used in LabSolutions GPC:

Term Description
Mn A number average molecular weight, which is calculated based on the total
number of molecules
Mw A weight average molecular weight, which is calculated based on the total
weight of molecules
Mz A Z average molecular weight
Mz1 A Z+1 average molecular weight
Mv A viscosity average molecular weight
Q factor A molecular weight per unit chain length of a polymer

2 LabSolutions GPC Instruction Manual

2 Starting the Analysis

This chapter describes the basic operation of LabSolutions GPC. For better understanding, it is
recommended that you actually operate LabSolutions GPC and work through the analysis procedure
of this chapter.
To specify the instrument control parameters for data acquisition and the peak integration
parameters, refer to "LabSolutions Operation manual". This manual primarily describes the
procedures particularly required for operating LabSolutions GPC.
2

2.1 Before Reading This Chapter

2.1.1 The Data Structure of LabSolutions GPC
This section gives a brief explanation about the data structure of LabSolutions GPC.
Adding to method parameters for LC Analysis and LC Postrun, the GPC data file also stores various data,
such as GPC calibration curve information and GPC calculation parameters.
This ensures the traceability of data since the analysis conditions and parameters can be referenced from
the data file itself. In addition, you can reanalyze the data using only the data file.

NOTE
The method information stored in the data file is the duplicate of the method file used during the acquisition
and analysis of data. Therefore, when modifying the method parameter in the data file, only the "method in
data" is modified and no changes are made to the original method file. But the original method file can be
updated accordingly with the modified method in the data file by using "Apply to the Method File" (save as a
method file) function.

2.1.2 LabSolutions GPC Operation Flow

In this chapter, the operation flow of LabSolutions GPC is explained as shown in the following chart.
In LabSolutions GPC, adding to the parameters for LabSolutions, you set parameters for calibration curve,
molecular weight calculation, etc. in a method file. You can create calibration curves in the [GPC Postrun]
program, and once you have set all these parameters, you can perform GPC calculation, such as
molecular weight calculation, while performing data acquisition by using the method file.

Explanation on how to view the following chart:

Operation
Application
Target files
(Their file name extensions)

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2 Starting the Analysis

File Names
File Names extension contents
Method file .lcm Analysis conditions, postrun analysis conditions, calibration
curve conditions
Report format file .lsr Report format
Batch file .lcb Batch table, batch settings
Data file .lcd Chromatogram, Peak table, Report format (Report format in
data), Method (copy), Batch table (copy)

Setting the analysis conditions

LabSolutions
[Realtime Analysis] - [Data Acquisition]
LabSolutions Method file (.lcm)

Analyzing standard sample

LabSolutions [Realtime Analysis] - [Data
Acquisition] or [Batch Processing]
LabSolutions Data file (.lcd), Batch file (.lcb)

Setting peak integration parameters

[GPC Postrun] - [GPC Data Analysis]
Modifying the method in the data file (.lcd)
Can modify the method file (.lcm)
Creating reports
accordingly
[GPC Postrun] - [Report Generator]
Creates a report format file (.lsr) for GPC
Creating calibration curves Postrun data.
[GPC Postrun] - [GPC Calibration Curve]
Creates a calibration curve in a method file
(.lcm) using data files (.lcd) of standard
samples.

Analyzing unknown sample

LabSolutions [Realtime Analysis] - [Data
Acquisition] or [Batch Processing]
Data file (.lcd), and Batch file (.lcb)

Viewing results
[GPC Postrun] - [GPC Data Analysis], and
[GPC Data Comparison]
Data file (.lcd)

Reanalyzing the results

[GPC Postrun] - [GPC Data Analysis] or
[Batch Processing]
Data file (.lcd)

4 LabSolutions GPC Instruction Manual

 2.2 Starting LabSolutions Analysis

NOTE
• In a batch table, the method file (.lcm) and the report format file (.lsr) are specified for each particular data
file (.lcd). When the batch analysis is completed, it creates a data file (.lcd) containing the same
information stored in the batch table (.lcb) and .lcm and .lsr (when a report is output) set for each line of
the batch table.
• When you open a method file (.lcm) in the [GPC Calibration Curve] window, the data files (.lcd) for all
levels in the calibration curve are loaded simultaneously. If the method file (.lcm) is edited here, the data
can be recalculated using the new analysis conditions for each data file (.lcd) that has been imported. You
can also optimize the quantitative method and the calibration curve type beside adding or deleting
calibration level data.
2
2.2 Starting LabSolutions Analysis

1 Display the [LabSolutions Main] window.

2 Click (Instruments).

3 Double-click on the instrument to use for analysis.

The [Realtime Analysis] program will start.

LabSolutions GPC Instruction Manual 5

2 Starting the Analysis

2.3 Preparing for Analysis

Referring to "LabSolutions Operation Manual", complete the system configuration and the preparation of
analytical instruments, and specify the instrument parameters as required. This section primarily explains
how to set parameters necessary for LabSolutions GPC analysis, such as the integration parameters, etc.
You can also set the parameters when creating calibration curves in the [GPC Postrun] program after
completing standard sample analysis.

2.3.1 Setting Data Analysis Parameters

1 Click [Data Processing Parameters] from the [Method] menu in the [Realtime Analysis]
program.

The [Data Processing Parameters] screen appears.

LabSolutions GPC performs the molecular weight calculation, etc., using "slice data". The "slice data"
refers to the height values obtained by separating chromatograms by a certain time interval. Therefore, in
addition to configuring the usual integration parameters in LabSolutions, the following settings must also
be configured in the [Data Processing Parameters] screen.

6 LabSolutions GPC Instruction Manual

 2.3 Preparing for Analysis

 Setting the Maximum Number of Slices

The maximum number of slices corresponds to the maximum number of "slice data" that can be stored in a
data file. You can set this value up to [50000].

1 Click [Advanced] in the [Integration] tab.

2 Enter the number of slices in [[Link]].

Set the maximum value: [50000].

NOTE
Each slice interval is 1/10 of the time (sec) specified as [Width] in the integration parameters. The
interval information is stored for each area detected as a peak. If a width of a detected peak is quite
long and the number of slices exceeds [50000], an error message appears. In this case, set the
larger value for [Width].

3 Click [OK].
This confirms the setting of the maximum number of slices.

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2 Starting the Analysis

 Setting [Link]
[Link] is a parameter that is used to automatically integrate peaks which broaden with time by increasing
[Width] and decreasing [Slope] according to the time specified.

1 Enter [1000] in [[Link]].

2 Click [OK].

NOTE
If [[Link]] is enabled in molecular weight distribution calculation, [Width] increases with time as a
function of the parameter. It follows the increase of intervals of slice data, and consequently the
molecular weight calculation may not be performed properly. To avoid this, you set [[Link]] to [1000]
to disable this parameter.

8 LabSolutions GPC Instruction Manual

 2.3 Preparing for Analysis

 Setting GPC Calculation Parameters

1 Click the [GPC Calculation] tab and configure the settings.

No. Description
Enter the element name. Up to 64 characters can be entered. The initial value is blank.
Enter the Q factor. A positive real number can be entered. The initial value is [1].
Enter the alpha value used for Mark Houwink conversion, etc. The initial value is [0].
(Alpha to be displayed for standard polymer are those specified in the [GPC Calibration Curve] tab.)
Enter the K value used for Mark Houwink conversion, etc. The initial value is [1].
(K to be displayed for standard polymer are those specified in the [GPC Calibration Curve] tab.)
Select whether you use the pump flow or a manually input value for the flow calculation. If you use a
manually input value, set the flow quantity per minute.
Select the molecular weight or the degree of polymerization for the display unit of the Y axis. [Molecular
weight] is selected initially. By selecting [Degree of Polymerization], you can enter [Molecular Weight]
per degree of polymerization.
Select a channel of the detector.
Specify whether to perform the GPC calculation for each channel.
Select a method of the time correction. [None] is selected initially.
Specify whether to perform the sensitivity compensation of the RI detector. When performing
compensation, click [Settings] and set the sensitivity compensation of the RI detector.
Click this to display the [RT/MW range setting] screen.

2 Click [OK].

Reference
"4.4.3 Setting Data Analysis Parameters"

NOTE
• Configure the settings in the [GPC Data Analysis] window or in the [GPC Calibration Curve]
window.
• If you have set these parameters in the [GPC Data Analysis] window, you need to apply the
"method inside data file" to the original method file by using "Apply to Method" function.
Also, you can create calibration curves in the [GPC Calibration Curve] window.

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2 Starting the Analysis

2.3.2 Saving Method Files

1 Click [Save Method File As] from the [File] menu.

2 Input the file name and click [Save].

NOTE
You can specify the default location for saving files by selecting the project folder in [File] -[Select
Project (Folder)].

2.4 Analyzing Standard Samples

When creating a calibration curve based on the analysis results of standard sample data, you need to
complete the data acquisition prior to the calibration curve creation. Referring to "LabSolutions Operation
Manual", analyze the standard sample through the single or batch analysis to acquire the sample data.

2.5 Creating Calibration Curve

In LabSolutions GPC, you create calibration curves in the [GPC Calibration Curve] window of the [GPC
Postrun] program. Prior to that, you need to set [Integration Parameters] and [GPC Calculation
Parameters].
This section describes how to create the calibration curve using the data already acquired.
The following is the brief explanation for the procedures:

10 LabSolutions GPC Instruction Manual

 2.5 Creating Calibration Curve

2.5.1 Displaying the [GPC Calibration Curve] Window

1 Display the [LabSolutions Main] window.

2 Click (Postrun).

3 Double-click (GPC Postrun).

The [GPC Postrun] program will start.

NOTE

• Click (GPC Postrun) for GPC data analysis, including molecular weight distribution

calculation, as you click (Postrun) for the regular data analysis.

• If (GPC Postrun) is not displayed on the menu, the license for LabSolutions GPC is not

recognized in your system. Check the authentication status of the software license.

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2 Starting the Analysis

4 Click
Postrun] program.
(GPC Calibration Curve) from the [Main] assistant bar in the [GPC

The [GPC Calibration Curve] window appears.

NOTE
When the [GPC Calibration Curve] window is displayed, no data has been loaded yet.

12 LabSolutions GPC Instruction Manual

 2.5 Creating Calibration Curve

2.5.2 Opening Method File

Open the method file used for analyzing the standard sample.

1 Click [Open Method File] from the [File] menu.

2
2 Select the method file and click [Open].

NOTE
You can select either LabSolutions method files or those from older software versions.

2.5.3 Displaying Data Files

1 Click [Data File List] from the [Data] menu.

LabSolutions GPC Instruction Manual 13

2 Starting the Analysis

2 Click [Add].

3 Select the standard sample data you wish to use for creating calibration curve, and click
[Open].

4 Verify that the data file of the standard sample is displayed in the data file list, and click
[OK].

NOTE
To select multiple sample data, repeat this procedure as necessary.

14 LabSolutions GPC Instruction Manual

 2.5 Creating Calibration Curve

2.5.4 Picking up Retention Time

1 Click [Edit] in the [Calibration Table View] screen.

2 Click [Pick up] in the [Calibration Table View] screen.

3 Click [All peaks] or [Select peaks].

Select [All peaks] when importing data of the whole retention time, and select [Select peaks] when
importing data for a certain time range. When [Select peaks] is selected, use the mouse to specify the
range on the chromatogram.

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2 Starting the Analysis

4 Click [New] or [Add].

Select [New] when importing peak data after deleting an existing calibration curve. Select [Add] when
adding peak data to existing calibration points.

5 Set the parameters not yet defined in the calibration curve table, such as molecular
weight values.

NOTE
• You can import parameter values from the [Table Edit] menu or by right-clicking and opening the
[Import Calibration Table] screen from the menu. When importing a method file, pick up the
[retention time] after importing. To do so, click [Retention Time Refresh] in the [GPC Method Edit]
screen.
• In the edit mode of Calibration Table View, you can delete unnecessary calibration points by
selecting the row of the calibration point and selecting [Delete Row] from the right-click menu.
• Peak data is sorted by the retention time when switching the edit mode to the view mode.

16 LabSolutions GPC Instruction Manual

 2.5 Creating Calibration Curve

2.5.5 Setting Calibration Curve Parameters

1 Select [Data Processing Parameters] from the [Method] menu.

2
2 Select the [Curve] type in the [GPC Calibration Curve] tab, and click [OK].

NOTE
If the number of calibration points is not enough, the calculation for high order calibration curve may
not be performed.

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2 Starting the Analysis

2.5.6 Saving Calibration Curves

1 Verify the calibration curve created in the [Calibration Curve View] screen.

NOTE
To modify the calibration curve, configure the settings again in the [Data Processing Parameters] -
[GPC Calibration Curve] tab.

2 Click [Save Method File] from the [File] menu.

The calibration curve is saved in the method file.

18 LabSolutions GPC Instruction Manual

 2.6 Creating Report Format

2.6 Creating Report Format

The procedures on creating a report format or summary report format of GPC analysis data are similar to
those for LabSolutions. Refer to "LabSolutions Operation Manual".
In LabSolutions GPC, the report items for outputting the results of GPC analysis, such as molecular weight
distributions, are newly added to the existing items. For more details, refer to "6 Report Function".

2.7 Analyzing Unknown Samples

This section describes the procedures for analyzing unknown samples through batch processing, using a 2
method file where the integration parameters and calibration curve are set in procedures "2.3 Preparing for
Analysis" and "2.5 Creating Calibration Curve".

2.7.1 Performing Batch Analysis

Set the schedule for continuous analysis.

1 Click

program.
(Realtime Batch) from the [Main] assistant bar in the [Realtime Analysis]

2 Select [New Batch File] from the [File] menu.

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2 Starting the Analysis

3 Enter the batch analysis schedule in the batch table.

The main items to be entered into the batch table are as follows:

Parameter Description
Vial# Enter the vial number of the sample injected by the autosampler. If no sample is being
injected, enter "-1".
Tray Name Enter the tray number of the sample injected by the autosampler. No entry is necessary
when using an autosampler without multiple tray settings.
Inj. Volume Enter the injection volume (unit: μL).
Sample Type Select the sample type.
In LabSolutions GPC, [GPC Control Sample] is added in the list in order to specify the
correction according to the control sample.
When you analyze an unknown sample first, enable [Initialize GPC Control] to initialize the
correction factor.

No. Description
The GPC Control Sample is used for correction of retention time.
Initializes the parameter for the time correction by GPC Control Sample.

Method File Specify the method file to be used for the analysis.
Data File Name the data that is to be saved as the analysis results.
When the file name is entered without a path, the data is created in the currently browsed
project folder (as previously specified).

NOTE
If an item is not displayed in the table, right-click on [Batch Table] and click [Table Style] from the
menu to set [Display Items].

4 Click (Save) in the toolbar, and save under a new name.

The content of the batch table is saved.

5 Click

NOTE
(Start) in the toolbar to start batch analysis.

The correction factor is not updated when the correction peaks of the control sample cannot be
identified. The control sample is corrected using the correction factor before the analysis.

20 LabSolutions GPC Instruction Manual

 2.8 Performing GPC Calculation

2.8 Performing GPC Calculation

This section describes how to view the results of analyzing molecular weight distribution of unknown
samples, etc., and how to set the GPC calculation parameters used for calculating molecular weight
distributions. By applying the GPC parameters optimized here to a method file and performing [Batch
Processing] in the [GPC Postrun] program, you can reanalyze multiple unknown sample data in the same
conditions.

2.8.1 Displaying the GPC Data Analysis Results

2
1 Click
program.
(GPC Data Analysis) from the [Main] assistant bar in the [GPC Postrun]

2 Click [Open Data File] from the [File] menu.

3 Select the data file, and click [Open].

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2 Starting the Analysis

The data file of unknown sample is loaded, and the chromatogram view displays the chromatogram, the
calibration curve, and the molecular weight distribution curve.

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 2.8 Performing GPC Calculation

2.8.2 Setting the Parameters for Molecular Weight Calculation

1 Select [Data Processing Parameters] from the [Method] menu.

2
2 Click the [GPC Calculation] tab to verify the parameters.

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2 Starting the Analysis

 Setting RT/MW Range

1 Click [RT/MW Range Settings] in the [GPC Calculation] tab.

2 Select [Time] or [M.W.], and input the RT/MW range.

3 Click [OK].

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 2.8 Performing GPC Calculation

2.8.3 Performing GPC Calculation

1 Click [OK] in the [Data Processing Parameters] screen.

The data is analyzed, and the chromatogram view displays the molecular weight distribution curve and
the integrated molecular weight distribution curve.

2 View the analysis results.

NOTE
When reanalyzing the data, click the [GPC Calculation] tab from the [Data Processing Parameters]
screen, and modify the calculation parameters.

2.8.4 Viewing Calculation Results

The calculation results for the slice data, peak table, average molecular weight table, and RT/MW range
table can be checked.

1 Click the appropriate item from the [View] menu.

2 Click the appropriate tab in the [Result Display] screen to check the contents.

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2 Starting the Analysis

2.9 Comparing GPC Data

You can overlay multiple results of GPC molecular weight calculation in one view, and also perform the
statistical calculation.

2.9.1 Displaying the [GPC Data Comparison] Window

1 Click

program.
(GPC Data Comparison) from the [Main] assistant bar in the [GPC Postrun]

NOTE
See "5 GPC Data Comparison" for details on the [GPC Data Comparison] window.

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 2.9 Comparing GPC Data

2 Drag and drop data files to compare from Data Explorer to the [GPC Data Comparison]
window view.

The chromatogams and molecular weight distribution curves appear.

NOTE
When different channels are used for GPC calculation in the drag-and-dropped files, the data file list
appears so that you can select a detector channel.

 Shifting Chromatograms
As you shift chromatograms, the molecular weight distribution curve and the integrated molecular weight
distribution curve shift accordingly.

1 Click [Shift Chromatogram Data] from the [Process] menu.

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2 Starting the Analysis

2 Configure the settings.

3 Click [OK].

NOTE

Chromatograms can be shifted as follows: Select the target data in the data list view, click (Move
Left/Right) in the chromatogram view, then drag the chromatogram.

 Statistical Calculation Results

1 Select [Statistical Calculation Results] from the [View] menu.

The [Statistical Calculation Result] screen appears.

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3 Generating Calibration
Curves
Calibration curves are registered in a method file by detector or channel. The molecular weight is
calculated besed on the eluent volume of analysis sample by a formula using the eluent volume and
the molecular weight of the standard sample.
To create calibration curves, check the [GPC Calculation] box on the [GPC Calculation] tab of the
[Data Processing Parameters] window.

3.1 Displaying the [GPC Calibration Curve] Window 3

1 Click
program.
(GPC Calibration Curve) in the [Main] assistant bar of the [GPC Postrun]

The [GPC Calibration Curve] window appears.

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3 Generating Calibration Curves

3.2 Using and Viewing the [GPC Calibration Curve] Window

The [GPC Calibration Curve] window has [Calibration Curve View], [Chromatogram View], and [Calibration
Table View]. (The areas sectioned by splitters (frames) are called "Views". You can customize the layout
and adjust the display area by dragging the splitters.)

[Calibration Curve View] [Calibration Table View]

[Chromatogram View]

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 3.3 Calibration Table View

3.3 Calibration Table View

In this view, you can set information about calibration points for creating a calibration curve.

No. Description
Click this to switch the calibration table into the view mode.
Click this to switch the calibration table into the edit mode.
Enter comments for the calibration curve. You can enter up to 64 characters.
Displays the setting of Mark Houwink conversion that is set in the data analysis parameters.
Displays the setting of time conversion that is set in the data analysis parameters.
Click this to import the peak data of the chromatogram displayed in the Chromatogram View into the calibration table.

3.3.1 Calibration Table

A calibration table displays information of calibration points in the table format. It can be entered and
modified only in the edit mode. The calibration point information includes the following items.

NOTE
If an item is not displayed in the table, right-click on [Calibration Table], click [Table Style] from the menu, and
set [Display Items].

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3 Generating Calibration Curves

Name Description
# Displays the numbers (1 - 64).
Time (min.) Enter the time of a calibration point (peak position). The value larger than the filtration limit
time should be entered. Enter the time after conversion when the time conversion is enabled.
Area% Displays the area% of calibration point. The area% is the value set in the [Settings Area%]
sub-window using the [Pick up] function. This item is displayed when Broad (Area%) is set for
Calibration Type.
Molecular Weight Enter the molecular weight of a calibration point (peak position). Enter the molecular weight
after the conversion when the Mark Houwink conversion is enabled.
Active Check this to make the calibration point active. If unchecked, the calibration point is not used
to calculate a calibration curve, and it is not displayed on the calibration curve graph in the
calibration curve view.
Virtual Check this to make the calibration point virtual. Virtual points are used as a supplement when
a calibration curve cannot be properly calculated using only the peak data.
Weight Select the weight from among [1000], [100], [10], [1], [1/10], [1/100], and [1/1000].
This can be entered when the [Weight] box is checked in the [Calibration Curve] tab of the
[Data Processing Parameters] screen.
Error (%) Displays errors between the calculated value of the calibration curve and the molecular
weight. An error is calculated by the following formula.
Error = (Molecular Weight - Calculated Value of Calibration Curve) * 100 / Molecular Weight
The following items display information about the peaks when the peak data is picked up from a chromatogram.
Values cannot be entered manually to these items in the table. [-] is displayed unless peak data is picked up.
Peak# Displays the peak numbers in a data file from which the time information is picked up.
Acquired by Displays who acquired the data file from which the time information is picked up.
Date Acquired Displays the data acquired date of the data file from which the time information is picked up.
Time Acquired Displays the data acquired time of the data file from which the time information is picked up.
Sample Name Displays [Sample Name] of the data file from which the time information is picked up.
Sample ID Displays [Sample ID] of the data file from which the time information is picked up.
Tray Name Displays [Tray Name] of the data file from which the time information is picked up.
Vial Number Displays [Vial Number] of the data file from which the time information is picked up.
Data File Displays [Data File Name] of the data file from which the time information is picked up.
Original Data File Displays [Original Data File] of the data file from which the time information is picked up.
(Full Path)
Original Method File Displays [Original Method File] of the data file from which the time information is picked up.
(Full Path)
Background Data File Displays [Background Data File] of the data file from which the time information is picked up.
Report Format File Displays [Report Format File] of the data file from which the time information is picked up.
Original Batch File Displays [Original Batch File] of the data file from which the time information is picked up.
(Full Path)
Description Displays [Description] of the data file from which the time information is picked up.
Processed by Displays [Processed by] of the data file from which the time information is picked up.
Date Processed Displays [Date Processed] of the data file from which the time information is picked up.
Time Processed Displays [Time Processed] of the data file from which the time information is picked up.

NOTE
When generating a calibration curve with chain lengths, enter chain lengths for molecular weights, and set
the Q factor correctly. When generating a calibration curve with molecular weights, set the Q factor to 1 (the
default value).

Reference
"4.4.3 Setting Data Analysis Parameters", " The Use of the Q Factor"

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 3.3 Calibration Table View

 Pop-up Menu
The following menus are displayed when right-clicking on the calibration table view. Displayed menus differ
when [Calibration Table View] is in the view or edit mode. Unavailable menu items are grayed.

In the view mode

Name Description
Copy Copies the selected cells into the clipboard.
Copy Entire Table Copies the entire table into the clipboard.
Select All Selects the entire table.
View Mode A check is displayed in front because the screen is in [View Mode].
Edit Mode Switches to the edit mode.
Export Calibration Table Outputs the calibration table into an ASCII file.
Table Style Allows setting the table style.
3
In the edit mode
The different menu items from the view mode are described.

Name Description
Pickup Peak Data Picks up the peak data from the data file into the calibration table.
All ON When the selected column is Active or Virtual, all the cells of that column are checked.
All OFF When the selected column contains checkboxes, all the cells are unchecked.
Cut Cuts the selected cells into clipboard.
Copy Copies the selected cells into the clipboard.
Paste Pastes the contents of the clipboard.
Clear Clears the selected cells.
Copy Entire Table Copies the entire table into the clipboard.
Select All Selects the entire table.
Add Row Adds a row at the end of the table.
Insert Row Inserts a row into the current cursor position.
Delete Row Deletes the selected rows.
Sort by Save Time Sorts the table contents by save time.
Data Analysis Parameters Displays the [Data Processing Parameters] dialog.
View Mode Switches to the view mode.
Edit Mode This item is preceded by a check mark because you are in the edit mode.
Cancel Edit Cancels the edit and switches to the view mode.
Import Calibration Table Imports calibration points from an ASCII file into the calibration table.
Export Calibration Table Outputs the calibration table to an ASCII file.
Table Style Allows you to configure the table style.

 Display Items and Their Order in the Calibration Table

The display items and the font of the calibration table can be set.

1 Right-click in the calibration table view and click [Table Style] from the menu.

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3 Generating Calibration Curves

2 Click the [Item Settings] tab, and set display items and their order.

No. Description
Shows items not displayed on the calibration table.
Shows items displayed on the calibration table. Items displayed here are displayed in the calibration
table starting from the left.
Moves items selected in [Hide Items] to [Display Items].
Moves items selected in [Display Items] to [Hide Items].
Moves the selected [Display Items] up or down.
Returns to the default for [Display Items] and [Hide Items].

3 Click the [Font] tab and set font.

No. Description
Enter or select a font name.
Enter or select a style.
Enter or select a size.
Select the text color.
Displays a text preview based on the configured settings.

4 Click [OK].

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 3.3 Calibration Table View

3.3.2 Importing Calibration Points from an ASCII File

You can import information including molecular weight and retention time into a calibration table from a file
that are saved in the ASCII format (such as the CSV format).

1 Click [Edit] in the calibration table view.

2 Right-click in the calibration table view and click [Import Calibration Table] from the
menu.
The [Import Calibration Curve Table] screen appears.

3 Specify the input file name and items to be imported.

3

No. Description
Enter the file name to be imported.
Click this to display the file selection screen.
Select items to be imported. Check the items to be imported from ASCII files and enter the column
number.
Select a delimiter for items in the input file.
Enter a delimiter when [Other] is selected for [Delimiter].
Specify the row number of the file to be imported where data start.
Click [OK] to import the specified file into [Calibration Table] using above settings.

4 Click [OK].
Calibration points are imported from the ASCII file based on the specified setting.

NOTE
The current content of [Calibration Table] is replaced with the content of the ASCII file.

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3.3.3 Exporting Calibration Table into an ASCII File

You can output the Items that have to be repeatedly entered, such as molecular weight, into an ASCII file
from the calibration table.

1 Click [Edit] in the calibration table view.

2 Right-click in the calibration table view and click [Export Calibration Table] from the
menu.
The [Export Calibration Curve Table] screen appears.

3 Set the name and items for the output file.

No. Description
Select where to output.
Enter an output file name when you selected [File Name].
Select items to be exported.
Select a delimiter for items in the output file.
Enter a delimiter when [Other] is selected for [Delimiter].

4 Specify an output file name and items to be output, and click [OK].
Data is output into an ASCII file from the calibration table based on the specified setting.

NOTE
Titles of the specified items are output in the first row. Values for Active and Virtual are in the ON/OFF
format.

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 3.3 Calibration Table View

3.3.4 Picking Up Peak Data from Chromatogram

You can pick up the peak data in a data file into [Calibration Table].

1 Click [Edit] in the calibration table view.

2 Click [Pick up] in [Calibration Table View] in [Edit] mode in the chromatogram view.
The peak data in a data file is copied into [Calibration Table].

Reference
"2.5.4 Picking up Retention Time"

3.3.5 Calculating Calibration Curve 3

When you switch the calibration table from the edit mode to the view mode, the content of the calibration
table is confirmed, and then the calibration curve is calculated.
The data is sorted by time, and then the calibration curve information is calculated.

1 Set information required for calculation of the calibration curve in the [Data Processing
Parameters] screen.

Reference
"3.7 Data analysis parameter"

2 Click [Edit] in the calibration table view.

3 Enter [Time], [Molecular Weight], [Active], [Virtual], and [Weight] in the calibration table.

4 Click [View] in the calibration table view.

The calibration table is confirmed, and the calibration curve information is calculated.

3.3.6 Creating a Calibration Point from Area%

A calibration point is created using the peak slice data for the peak, and the correspondence table for the
known molecular weight and area% for the peak.

1 Change the setting to [Broad (Area%)] at [Calibration Type] on the [GPC Calibration
Curve] tab in the [Data Processing Parameters] screen.

Reference
"3.7 Data analysis parameter"

2 Set the correspondence table for the molecular weight and area% in the [Settings
Area%] sub-window.

Reference
"3.7 Data analysis parameter"" Setting Area%"

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3 Generating Calibration Curves

3 Load the data files.

Reference
"3.5.1 Data File List"" Adding Data Files"

4 Import the slice data for the peak.

Click [Edit] in [Calibration Table View] and then click [Pick Up].

5 Select [First Peak] when importing the slice data for the initial peak. Select [Select Peak]
when specifying a peak to import.
When [Select Peak] is selected, click a peak to import in the chromatogram.

6 Select [New] to discard the existing calibration curve and import a new calibration point.
Select [Add] when adding it to existing calibration points.

7 Save the calibration curve.

Reference
"2.5.6 Saving Calibration Curves"

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 3.3 Calibration Table View

3.3.7 Creating a Calibration Point from two known molecular weights

Calculate a linear calibration curve using the peak slice data and two known average molecular weights to
create a calibration point.

1 Change the setting to [Broad (Hamielec)] at [Calibration Type] on the [GPC Calibration
Curve] tab in the [Data Processing Parameters] screen.

Reference
"3.7 Data analysis parameter"

2 Set the average molecular weight information used to determine the linear calibration
curve in the [Molecular Weight Settings] sub-window. 3
Reference
"3.7 Data analysis parameter"" Setting Molecular Weight"

3 Load the data files.

Reference
"3.5.1 Data File List""■ Adding Data Files"

4 Import the slice data for the peak.

Click [Edit] in [Calibration Table View] and then click [Pick Up].

5 Select [First Peak] when importing the slice data for the initial peak. Select [Select Peak]
when specifying a peak to import.
When [Select Peak] is selected, click a peak to import in the chromatogram.

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3 Generating Calibration Curves

6 Set the table ID and click on [New] or [Add].

The calibration point is calculated by setting the Table ID to the corresponding Table ID on the [Molecular
Weight Settings] sub-window. Select [New] when discarding the existing calibration curve and importing
new calibration points and select [Add] when adding to existing calibration points.

7 Save the calibration curve.

Reference
"2.5.6 Saving Calibration Curves"

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 3.4 Calibration Curve View

3.4 Calibration Curve View

Displays calibration curves registered in method files.

3
No. Description
Displays factors and identity (R2, R, Dispersion) of the calibration curve.
Displays the time or elution volume and log(M.W.) of the coordinates in real time when placing the mouse on
the calibration curve graph.
The time and the elution volume can be switched by the axis configuration of the graph.

3.4.1 Calibration Curve Graph

A calibration curve is displayed in this graph.

Calibration curve graph legend

 : Calibration point
: Calibration point (Average value [Note])
 : Calibration point (Virtual)
Filtration limit value line (Default shown in blue)

NOTE
This is a calibration point of the molecular weight of which the average is calculated when [Time is averaged
for the same M.W.] on the [GPC Calibration Curve] tab in the [Data Analysis Parameters] screen is checked.

Zooming in graph

When specifying a range by dragging a square on the calibration curve graph, the graph is zoomed in
according to the specified range.

Adding virtual points

Double-click on the calibration curve graph to register the coordinate in the calibration table as a virtual
point.

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3 Generating Calibration Curves

Right-click menu

Right-click on the calibration curve graph to display the menu.

Name Description
Undo Zoom Cancels the previous zooming action.
Redo Zoom Zoom again.
Initialize Zoom Returns the display to the initial state.
Data Processing Displays the calibration curve tab of the [Data Processing Parameters] screen.
Parameters
Copy Copies the calibration curve graph image to the clipboard.
Scale Settings Displays the [Scale Setting] screen.
Properties Sets display attributes such as graph color.

 Setting the Scale of the Graph

Set the scale of the calibration curve graph.

1 Right-click on the calibration curve graph and click [Scale Settings] from the menu.

2 Configure the settings.

No. Description
Select between [Autoscale] and [User Defined] for the scale of the calibration curve graph.
Enter the X axis range of the calibration curve graph.
Enter the Y axis range of the calibration curve graph.

3 Click [OK].

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 3.5 Chromatogram View

3.5 Chromatogram View

This view displays chromatograms of data files used to create a calibration curve. The data files are
registered in the data file list. The chromatogram view has the following three tabs.

3
No. Description
Displays a chromatogram of the selected data.
Overlays all chromatograms in the data file list.
Displays sample information of the selected data.

3.5.1 Data File List

Data files used when creating calibration curves are displayed in a list. Data files can be added or deleted.

1 Right-click on the chromatogram and click [Data File List] from the menu.

2 Configure the settings.

No. Description
Lists the data files registered in the method file.
Click a file name to make the file selected.
Adding a data file.
Click this button to delete the registration of the data file selected in the data file list.

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3 Generating Calibration Curves

3 Click [OK].

NOTE
Select [View] - [Full Path] from the menu on the [Calibration Curve] screen to display file names in full path.

 Adding Data Files

Data files to create the calibration curve are added in the data file list.

1 Click [Add] on the [Calibration Curve] screen.

The [Add Data File] screen appears.

2 Select data file to be added, and click [Open].

The selected data file is added to the data file list.

NOTE
Data files can be added if you drag-and-drop them from Data Explorer into the chromatogram view.

 Deleting Data Files

Data files to create the calibration curve are deleted from the data file list.

1 Select a data file to be deleted from the data file list.

2 Click [Delete].
The message [Do you remove data file from data file list?] appears.

3 Click [OK].
The selected data file is deleted from the data file list.

NOTE
When swiching file name display, adding or deleting data files, clicking [OK] and closing the
[Calibration Curve] screen reflect the change.

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 3.5 Chromatogram View

3.5.2 [Single] Tab

This tab displays an entire chromatogram of the data file selected from the data files used to create the
calibration curve.

No. Description
Select a data file to display.

 Zooming the Graph

3
When specifying a range by dragging a square on the chromatogram, the graph is zoomed in according to
the specified range.

 Displaying the Right-click Menu

Right-click on the chromatogram to display the menu.
The displayed menu is as follows:

Name Description
Undo Zoom Cancels the previous zooming action.
Redo Zoom Zoom again.
Initialize Zoom Returns the display to the initial state.
Peak Table Displays the peak table of the selected data file.
Manual Integration Bar Displays or hides the manual peak integration bar.
Data File List Displays the [Calibration Curve] screen.
Copy Copies the chromatogram image to the clipboard.
Properties Sets display attributes such as graph color.

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3 Generating Calibration Curves

3.5.3 [Multi] Tab

Multiple data files registered in the data file list are overlayed in one graph.

 Zooming the Graph

When specifying a range with the mouse on the chromatogram, graph is zoomed in according to the
specified range.

 Displaying the Right-click Menu

Right-click on the chromatogram to display the menu.
The displayed menu is as follows:

Name Description
Undo Zoom Cancels the previous zooming action.
Redo Zoom Zoom again.
Initialize Zoom Returns the display to the initial state.
Base Shift Displays or hides the base shift.
Data File List Displays the [Calibration Curve] screen.
Copy Copies the chromatogram image to the clipboard.
Properties Sets display attributes such as graph color.

3.5.4 [Sample Info.] Tab

Sample information of data files is displayed.

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 3.6 Peak table

3.6 Peak table

Peak table of the selected data in the data file list is displayed.

1 Right-click on the chromatogram and click [Peak Table] from the menu.
The peak table is displayed.

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3 Generating Calibration Curves

3.7 Data analysis parameter

Parameters related to calculations of calibration curve or molecular weight are set.

1 Click [Data Processing Parameters] from the [Method] menu.

2 Click the [GPC Calibration Curve] tab, and configure the settings.

No. Description
Select a channel to set the calibration curve information.
Select the calibration type.
Select a calibration curve type.
Enter the filtration limit time.
Check this box to enable the weight setting so that calibration curves are weighted in the calculation.
Check this box to calculate an average value of times of calibration points having the same
molecular weight in the calibration table and treat it as a single calibration point.
Displays the [Area% Settings] sub-window. This item is displayed when [Broad (Area%)] is set at
[Calibration Type].
Check this box to enable the Mark-Houwink conversion.
Click this button to open the setting screen for the time conversion.
Check this box to enable the time conversion.
Set parameters used in Mark-Houwink conversion.

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 3.7 Data analysis parameter

 Setting Mark Houwink Conversion

1 Check [Mark-Houwink] on the [GPC Calibration Curve] tab.

2 Set the [Mark-Houwink Conversion Parameters] fields.

No. Description 3
Set the alpha and K of the standard polymer
Displays the alpha and K of the unknown polymer.
(Values set in [GPC Calculation] tab in Data Processing Parameters are fixedly displayed.)

3 Click [OK].

NOTE
Mark Houwink conversion is performed with the following formula.
logMb = (1 / (1 + αb)) log (Ka / Kb) + ((1 + αa) / (1 + αb)) logMa

Ma: Molecular weight of the standard polymer

Mb: Molecular weight of the unknown polymer
αa: Alpha of the standard polymer
αb: Alpha of the unknown polymer
Ka : K of the standard polymer
Kb : K of the unknown polymer

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3 Generating Calibration Curves

 Setting Time Conversion

1 Check [Time Conversion] in the [GPC Calibration Curve] tab, and click [Settings].

2 Configure the settings.

No. Description
Enter time before conversion.
Enter time after conversion.

3 Click [OK].

NOTE
Time conversion is performed with the following formula.
RTc = (time after conversion / time before conversion) RT

 Setting Area%

1 Select [Broad (Area%)] at [Calibration Type] in the [GPC Calibration Curve] tab and click
[Area% Settings].

2 Configure the settings.

No. Description
This is the correspondence table for molecular weight and area%.
Up to 100 rows can be entered.

3 Click [OK].

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 3.7 Data analysis parameter

 Setting Molecular Weight

1 Select [Broad (Hamielec)] at [Calibration Type] in the [GPC Calibration Curve] tab and
click [Avg. M.W. Settings].

2 Configure the settings.

No. Description
Specify the average molecular weight to be used for determining the linear calibration curve.
Enter the average molecular weight for the specified average molecular weight when "Enter the
molecular weight" is selected.

NOTE
• [Enter the molecular weight] cannot be specified for peak top molecular weight.
• For items other than peak top molecular weight, at least two settings other than [Do not use] are
required.
• The table ID must be a value between 0 and 100. It will be referenced in the [Add Peak] screen.
• The average molecular weight should be set so that Mn ≤ Mw ≤ Mz ≤ Mz + 1 .

3 Click [OK].

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52 LabSolutions GPC Instruction Manual

4 GPC Calculation

This chapter explains the GPC calculation.

In the GPC calculation, a molecular weight distribution and an average molecular weight of samples
are calculated from acquired chromatograms and calibration curves.

4.1 Displaying the [GPC Data Analysis] Window

1 Click the

Postrun] program.
(GPC Data Analysis) icon in the [Main] assistant bar of the [GPC

The [GPC Data Analysis] window appears.

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4 GPC Calculation

4.2 Using and Viewing the [GPC Data Analysis] Window

The [GPC Data Analysis] window has [Chromatogram View] and [Molecular Weight Distribution View].
[Chromatogram View]

[Molecular Weight Distribution View]

No. Description
Chromatograms and calibration curves are displayed.
Differential and integral molecular weight curves calculated from the chromatogram and the calibration curve
are displayed.
Click this button (change size button) to switch between the full screen and standard displays.

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 4.3 Chromatogram View

4.3 Chromatogram View

In [Chromatogram View], the chromatogram and the calibration curve are displayed in layers.

4
No. Description
Displays the full chromatogram. An area to be zoomed in can be specified by dragging a mouse.
Zooms into a specified area on the chromatogram. An area to be zoomed into can be specified by dragging a
mouse.
Displays the sample information.

 Right-click Menu
When right-clicking on the chromatogram, a menu appears. You can select various operations and settings
for the chromatogram from the displayed menu.

4.3.1 Display Settings of Chromatograms

 Display of Chromatograms
Set the display of the chromatograph.

1 Right-click on the chromatogram, and click [Display Settings] from the displayed menu.

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4 GPC Calculation

2 Click the [Chromatogram] tab and set the display of the chromatogram.

No. Description
Displays or hides Ch1 through Ch4 chromatograms when the detector is of the dual mode or the PDA detector.
Displays or hides the base shift.
Set the intensity axis unit. The contents and default values displayed in the list vary according to the detector.
Displays or hides the full chromatogram.
Set the height of the full chromatogram on the screen.
When checked, the chromatogram is displayed by adjusting the scale of the intensity axis to 0 V or more.
Select the chromatogram scale on the intensity axis. [Auto-scale to Largest Peak] is selected initially.
When selecting [User Defined], the [Intensity Range] can be set.
When checked, the intensity axis range displayed for all data will be fixed to the set value.
Sets the zoom chromatograms to either an overlaid display or a stacked display.
Displays or hides the chromatogram of another detector.
When checked, select the other detector.
Allows you to set the upper and lower limits of the intensity axis of the zoom chromatogram for each detector.
When this button is pressed, the display range of the current graph is applied to the range of the zoom
chromatograms.
Set the upper and lower limits of the time axis of the zoom chromatogram.
Fixes the time axis range displayed for all data to the set value or releases it.
Fixes the intensity axis range displayed for all data to the set value or releases it.
Displays or hides the GPC calibration curve.
Select the GPC calibration curve scale on the intensity axis.
When selecting [User Defined], the [Molecular Weight Range] can be set.
h Displays or hides the calibration points.

NOTE
The range of the horizontal axis of the zoom chromatogram is specified with (min) when the display unit is
time, and with (mL) when it is the eluent volume.

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 Display of Status Graphs

Sets the display of the status graphs.

1 Right-click on the chromatogram and click [Display Settings] from the menu.

2 Click the [Status] tab, and set the display of the status graphs.

No. Description
Displays or hides the sample information.
Check items to draw the status curve.
Set the upper and lower limits of the temperature. Click [Normalize] to set the temperature range
automatically.
Set the upper and lower limits of the pressure. Click [Normalize] to set the pressure range automatically.
Set the upper and lower limits of the flow. Click [Normalize] to set the flow range automatically.
Set the upper and lower limits of the concentration. Click [Normalize] to set the concentration range
automatically.
Set the upper and lower limits of the light intensity. Click [Normalize] to set the light intensity range
automatically.
Set the upper and lower limits of the pH. Click [Normalize] to set the pH range automatically.
Select the unit of measure for the intensity axis on the right-hand side.

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4.3.2 Setting View Properties

1 Right-click on the chromatogram and click [Graph View Properties] from the menu.

2 Click the [General] tab and configure the settings.

No. Description
Displays or hides the X axis grid.
Displays or hides the Y axis grid.
Displays or hides the Y axis intensity value in the exponential format (such as x10 or x100).
Displays or hides the graph comment.
Select either [M.W./D.P] or [log (M.W.) /log (D.P.)] for the scale display when displaying the molecular
weight information (molecular weight or degree of polymerization) in a graph comment.
When [Show Mouse Position] is checked, the Y-axis coordinate of the mouse will be displayed in the
intensity value.
Displays or hides the magnification buttons (zoom-in and zoom-out).
Displays or hides the Y axis scroll bar.
Displays or hides the detector and channel names.
Displays or hides the extra curve labels, such as of temperature and pressure.
Displays or hides the peak mark, baseline, fraction collector result and simulation.
Select items to display as peak top comments.

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3 Click the [Color] tab and configure the settings.

No. Description
Select a display item for color setting.
Displays the currently set colors.
Click these to set the color of the display item.
Click these to Initialize the color of the display item.

4
4 Click the [Font] tab and configure the settings.

No. Description
Allows you to select an item for the font setting.
Displays the selected [Font Name], [Font Size], and [Font Style].
Displays the [Font] screen for selecting the font.

NOTE
The X axis of chromatogram is displayed in time (min) or eluent volume (mL) depending on the
setting. The Y axis of chromatogram is displayed in log of the molecular weight (M.W.) or log of the
degree of polymerization (D.P.) depending on the setting.

5 Click [OK].

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4.4 Molecular Weight Distribution View

In [Molecular Weight Distribution View], the molecular weight distribution curves calculated from the
chromatograms and the calibration curves are displayed. There are two types of molecular weight
distribution curves: differential and integral.

No. Description
Displays molecular weight distribution curves.
Displays the correction factor.

4.4.1 Display Settings of the Molecular Weight Distribution Curves

1 Right-click on the molecular weight distribution curve graph and click [Display Settings]
from the menu.

2 Configure the settings.

No. Description
Select either [Overlay] or [Stack] for the display of the molecular weight distribution graphs (differential
and integral). These displays are available for multiple detectors or a detector supporting the dual mode.
Displays or hides the integral molecular weight distribution.

3 Click [OK].

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4.4.2 Setting Properties of Molecular Weight Distribution Curve

1 Right-click on the molecular weight distribution curve graph and click [Properties] from
the menu.

2 Click the [General] tab and configure the settings.

No. Description
Displays or hides the X axis grid.
Displays or hides the Y axis grid.
Switches the display of the Y axis intensity value in the exponential format (such as x10 or x100).
Displays or hides the magnification buttons (zoom-in and zoom-out).
Displays or hides the Y axis scroll bar.
Displays or hides the graph comment.
Select either [M.W./D.P] or [log (M.W.) /log (D.P.)] for the scale display when displaying the molecular
weight information (molecular weight or degree of polymerization) in a graph comment.
When [Display Y-axis value at intensity.] is checked, the Y-axis coordinate of the mouse will be displayed
in the intensity value.
Select whether to display the average molecular weight mark for the entire graph or for each peak.
Select the items to be displayed.

NOTE
The X axis of the molecular weight distribution graph is displayed in the molecular weight log(M.W.)
or the degree of polymerization log(D.P.) depending on the setting.

3 Click the [Color] tab and configure the settings.

Reference
For details, see step 3 (P.59) in "4.3.2 Setting View Properties".

4 Click the [Font] tab and configure the settings.

Reference
For details, see step 4 (P.59) in "4.3.2 Setting View Properties".

5 Click [OK].

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4.4.3 Setting Data Analysis Parameters

Molecular weight distribution is calculated and displayed by setting the data analysis parameters.
Set the following parameters:
• Parameters for samples (Q factor, alpha value, K value)
• Data for GPC calculation (detector, channel)
• Whether or not to perform time correction, its type and necessary factors
• Sensitivity compensation of the RI detector

1 Click [Data Processing Parameters] from the [Method] menu.

2 Click the [GPC Calculation] tab and configure the settings.

No. Description
Enter the element name. Up to 64 characters can be entered. The initial value is blank.
Enter the Q factor. A positive real number can be entered. The initial value is 1.
Enter the alpha value used for Mark Houwink conversion, etc. The initial value is 0.
(Alpha to be displayed for standard polymer are those specified in the [GPC Calibration Curve] tab.)
Enter the K value used for Mark Houwink conversion, etc. The initial value is 1.
(K to be displayed for standard polymer are those specified in the [GPC Calibration Curve] tab.)
Select whether you use the pump flow or a manually input value for the flow calculation. If you use a
manually input value, set the flow quantity per minute.
Select the molecular weight or the degree of polymerization for the display unit of the Y axis. [Molecular
weight] is selected initially. By selecting [Degree of Polymerization], you can enter the molecular weight
per degree of polymerization.
Select a channel of the detector.
Specify whether to perform the GPC calculation for each channel.
Select a method of the time correction. [None] is selected initially.
Specify whether to perform the sensitivity compensation of the RI detector. When performing
compensation, click [Settings], and set the sensitivity compensation of the RI detector.
Click this to display the [RT/MW Range Setting] screen. Enter the start and end time.

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 The Use of the Q Factor

• What is Q factor?
In GPC, components are separated based on the molecular size and a relationship between the elution
position and the molecular size is set by a formula. When equations differ between the standard sample
(from which a calibration curve is created) and the unknown sample (for which you want to find out the
molecular weight), correction is required to obtain accurate molecular weight. The Q factor is used in such
case.

• How to use the Q factor

Q factor is "molecular weight of each monomer divided by the stretched chain length of each monomer".
When this value is set, GPC data analysis, such as molecular weight distribution calculation, is done
using stretched chain length in LabSolutions. Using the default value of 1 means that the calculation is
done using molecular weight.

• Notational reminder for the use of Q factor

When you use the conversion with the Q factor, you need to interpret the values as [Stretched Chain
Length] even though they are discrbed as [Molecular Weight] in the following items in LabSolutions.
- Molecuar weight in a calibration curve table
4
- Molecuar weight in a slice data
- Molecuar weight in a calibration curve graph
- Molecuar weight in a molecular weight distribution curve
Only average molecular weights displayed in a GPC calculation result are expressed in molecular weight
that is corrected using the Q factor, not in stretched chain length.

• Reminder on setting when using the Q factor

As explained above, you need interpret values described as molecular weight in a calibration curve table
as stretched chain length when you use correction with the Q factor. Therefore, specify strethced
molecular chain length values in the molecular weight cells when creating a calibration curve table.

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 Setting the Time Correction

Set the time correction to compensate for variations in the molecular weight distribution calculation due to
changes in elution time.

1 Check [Internal Standard] or [GPC Control sample] in [Time Correction], and click
[Settings].

2 Configure the settings.

No. Description
Set the retention time of the standard peak used for the time correction.
Standard peak refers to the internal standard peak when using "Internal Standard Method". When using
"GPC Control Sample", it refers to the peak for data whose batch processing sample type is set to “GPC
Control Sample”.
Set the band of the standard peak.
Set the base time (T0) used for the time correction calculation. Usually, zero or [Link] (filtration limit
time) is set.
Select whether to also calculate the internal standard peak in the molecular weight calculation.

3 Click [OK].

Reference
"4.5.5 Time Correction Using the Internal Standard Method" on page 74

"4.5.6 Time Correction Using the Control Sample" on page 75

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 Sensitivity Compensation Setting of RI Detectors

Since the RI detector has a characteristic that the sensitivity lowers if the molecular weight of analyzed
sample is low, perform the sensitivity compensation by setting the sensitivity compensation parameters.
To use this function, it is necessary to ascertain the relationship between the molecular weight and the
response sensitivity (area) as described in "4.5.7 Sensitivity Compensation of RI Detectors".

1 Check [Sensitivity Compensation (RID)] and click [Settings].

2 Configure the settings.

No. Description
Set the base area. Set a value equal to or greater than the maximum value of the sensitivity
compensation table area.
The area value correction factor is calculated with the following:
• Correction factor = (base area) / (Area value of the molecular weight on the graph)
This is the mapping table of the molecular weight and the area values. A maximum of 20 lines can be
entered and must monotonically increase.
When clicked, the contents of the sensitivity compensation table are drawn in the graph in .
Displays the sensitivity compensation graph.

3 Click [OK].

NOTE
The table contents can be copied and cut using the right-click menu.

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 Setting the RT/MW Range

Specify ranges for performing GPC calculation.
• You can specify multiple (up to 20) ranges in the chromatogram or the molecular weight distribution graph.
• You can specify time (molecular weight) by entering numbers or clicking on the graph.
• You can specify a name for the section.

1 Click [RT/MW Range Setting].

The [RT/MW Range Setting] screen appears.

2 Configure the settings.

No. Description
Switches the graph to the chromatogram or the molecular weight distribution curve.
Displays the chromatogram or the molecular weight distribution curve.
Displays the set RT/MW ranges. Enter values in [Start Time] and [End Time] or [Min. Molecular Weight]
and [Max. Molecular Weight] to specify the RT/MW range settings. A name can also be set for any
range.

3 Click [OK].

NOTE
The table contents can be copied and cut using the right-click menu.

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4.4.4 Displaying Slice Data

The slice data, an intermediate step of the molecular weight calculation, is displayed.
• The slice information of the specified peak is displayed at a specified interval.
• Items to be displayed and their order can be customized in the table style setting.
• Selected items can be transferred to the clipboard.

1 Click [Slice Data] from the [View] menu.

2 Configure the settings.

No. Description
Select a channel of the detector.
Select [Total] or any of the peak numbers.
Specify an interval to display the slice data.
Displays data of each slice at the specified interval.

3 Click [Close].

NOTE
• [Slice Data] can be selected from the [View] menu only when [Calculate GPC] is checked in the [GPC
Calculation] tab of the [Data Processing Parameters] screen.
• Changing the output interval ([Steps]) of the slice data does not alter the numbers displayed in the
left-most [#] column.

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 Setting the Table Style

The table style of the slice data is set as follows:

1 Right-click on the data table in the [Slice Data] tab, and click [Table Style] from the
menu.

2 Click the [Item Settings] tab and configure the settings.

No. Description
Hidden items are displayed.
Displayed items are displayed.
Moves items selected in [Hide Items] to [Display Items].
Moves items selected in [Display Items] to [Hide Items].
Click these to move a selected item in [Display Items] up or down.
Returns [Display Items] and [Hide Items] to their default status.

3 Click the [Font] tab and configure the settings.

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No. Description
Enter or select a font name.
Enter or select a font style.
Enter or select a font size.
Select a text color.
Character display sample based on the setting is displayed.

4.4.5 Displaying the Results from the Peak Table

The peak information or the detailed information for individual peaks are displayed.
• The peak information (retention time, height, etc.) is listed.
• Detailed information (values at the start, top, and end) of the selected peak is displayed.
• Items to be displayed and their order can be customized in the table style setting.
• Selected items can be transferred to the clipboard.

1 Click [Peak Table] from the [View] menu. 4

2 Check the items.

No. Description
Lists the peak information of the currently selected detectors and channels.
Displays the detailed information of the selected peak.

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 Setting the Table Style

Reference
"4.4.4 Displaying Slice Data", " Setting the Table Style"

NOTE
Operations are the same for any table, but the displayed items and hidden items are different.

4.4.6 Displaying the Result of the Average Molecular Weight

The average molecular weight is displayed by each peak.
• Average molecular weight of all the peaks and of individual peaks can be calculated.
• Items to be displayed and their order can be customized in the table style setting.
• Selected items can be transferred to the clipboard.

1 Click [Molecular Weight Distribution Table] from the [View] menu.

2 Check the items.

The average molecular weight, the polydispersity, and the intrinsic viscosity of the all the peaks and of individual
peaks is listed. Items to be displayed and their order can be customized in the table style setting.

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 Setting the Table Style

Reference
"4.4.4 Displaying Slice Data", " Setting the Table Style"

NOTE
Operations are the same for any table, but the displayed items and hidden items are different.

4.4.7 Displaying the Result of the Average Molecular Weight of the RT/MW Ranges
The average molecular weight values of the RT/MW ranges are displayed.
• Average molecular weight can be calculated for each specified RT/MW range.
• Items to be displayed and their order can be customized in the table style setting.
• Selected items can be transferred to the clipboard.

1 Click [RT/MW Table] from the [View] menu.

4

2 Check the items.

The average molecular weight, the polydispersity, and the intrinsic viscosity of each RT/MW range is listed.
Items to be displayed and their order can be customized in the table style setting.

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 Setting the Table Style

Reference
"4.4.4 Displaying Slice Data", " Setting the Table Style"

NOTE
Operations are the same for any table, but the displayed items and hidden items are different.

4.5 GPC Calculation

A chromatogram is converted into a molecular weight distribution using an approximate equation, and
various average molecular weight values are calculated from the molecular weight distribution.
The following time corrections are performed in the conversion to the molecular weight distribution.
• Correction using the internal standard method
• Correction using the control sample
• Sensitivity compensation of RI detectors

4.5.1 Creating a Calibration Curve Table

Use the following method to create a calibration curve table.

Calibration Type Creating a Calibration Curve Table

Normal Select a peak using the [Pick up] function.
The retention time for the peak, and the calibration point for undetermined
molecular weight are added to the calibration curve table.
Complete the calibration curve table by setting the molecular weight
corresponding to each calibration point.
Broad (Area%) Select a peak using the [Pick up] function.
The area% for the peak at each retention time is calculated from the slice data for
the selected peak.
For the pair of molecular weight and area% set in the [Area% Settings] sub-
window, the software searches for the slice data with the closest area%.
In addition, calibration points are created using the set molecular weight and
retention times of the found slice data.
Broad (Hamielec) Select a peak using the [Pick up] function.
A linear calibration curve is determined from the slice data of the selected peak
and the two average molecular weights specified on the [Average Molecular
Weight Settings] sub-window. Retention times are calculated from the linear
calibration curve and the average molecular weights used in the calculations,
creating calibration points.

NOTE
When Broad (Hamielec) is selected, LabSolutions determines the linear calibration curve using the following
calculation method.

The linear calibration curve is given by Equation (1).

Calculates the coefficients a and b.
log M i = aT i + b ... (1)
The calibration curve in Equation (1) can be transformed as follows:

M i = 10 aT i + b = 10 b ⋅ 10 aTi ... (2)

Substitute Equation (2) into Mw ⁄ Mn .

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 4.5 GPC Calculation

Hi
 ( Mi ⋅ Hi ) ⋅  ------
Mi
( Mw ⁄ Mn ) = ------------------------------------------------
2
(  ( Hi ) )

Hi
 ( 10 aTi ⋅ Hi ) ⋅  ------------
10 aTi
-
= ------------------------------------------------------------- ... (3)
2
(  ( Hi ) ) (Hi: Sample conc. / Mi: Molecular weight / Ti: Time)

The coefficient a is determined using a iterative method calculation method, and then the coefficient b is
calculated using the coefficient a.

4.5.2 Approximate Equations for Calibration Curves

The following approximate equations are used for obtaining molecular weights from the chromatograms.

Type Equation
Point-to-point
Linear (Straight line)
Linear interpolation between calibration points
log M = aX + b
4
3rd order log M = aX3 + bX2 + cX + d
3rd order + Hyperbolic curve log M = aX3 + bX2 + cX + d + e/X2
5th order log M = aX5 + bX4 + cX3 + dX2 + eX + f
5th order + Hyperbolic curve log M = aX5 + bX4 + cX3 + dX2 + eX + f + g/X2
7th order log M = aX7 + bX6 +cX5 + dX4 + eX3 + fX2 + gX + h
7th order + Hyperbolic curve log M = aX7 + bX6 +cX5 + dX4 + eX3 + fX2 + gX + h + i/X2

X = x(elution duration) - [Link](filtration limit time)

M: the molecular weight or the chain length

4.5.3 Equations for Calculating Average Molecular Weight

Type Equation
Number average Mn ΣHi
Mn = ×QF
Σ(Hi/Mi)
Weight average Mw Σ(Mi × Hi)
Mw = ×QF
ΣHi
Z Average Mz Σ(Mi2 × Hi)
Mz = ×QF
Σ(Mi × Hi)
Z+1 Average Mz1 Σ(Mi3 × Hi)
Mz1 = ×QF
Σ(Mi2 × Hi)

( )
Viscosity average Mv
Mv =
Σ(Hi × Mi α) 1/α
×QF
ΣHi
Intrinsic viscosity [Link] = K × (Mv)α

Hi: Peak height

Mi: Molecular weight or chain length
α/K : Constant and Index of the viscosity equation
QF: Q factor (1 for molecular weight)

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4.5.4 Time Correction

The following time correction can be performed in conversion to the molecular weight distribution:
• Correction using the internal standard method
• Correction using the control sample

4.5.5 Time Correction Using the Internal Standard Method

A chromatogram is measured after adding the internal standard sample to the unknown sample that is to be measured.
The time axis of the actual chromatogram is corrected using the peak of the internal standard sample of which retention
time (Tis) is known. The corrected chromatograms are expanded or reduced along the time axis.
[Before correction]
t1
Internal standard peak

tis

t
Standard time T0 Set time Tis
[After correction]
Band
Tis - T0
T0 + × (t1-T0)
tis - T0

Tis

NOTE
Time before base time T0 is not corrected.

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4.5.6 Time Correction Using the Control Sample

In this method, analysis is done using samples at the internal standard peaks as the GPC control samples.
As shown in the following figure, GPC control samples are injected among unknown samples in a batch
schedule, and the correction values are continually updated.

Schedule

GPC control Unknown Unknown GPC control Unknown Unknown

sample sample sample sample sample sample

㫋㪼㫊㪈 㫋㪼㫊㪉

㫋㪼㫊
Peak of control sample

GPC control

4
sample

㪙㪸㫊㪼㩷㫋㫀㫄㪼㩷㪫㪇 㪫㪼㫊㩷Set time

㫋㪈

Before correction
(Unknown sample)
㫋㪉
㪫㪼㫊㩷㪄㩷㪫㪇
㫋㪉㩷㪔㩷㪫㪇㩷㪂 㬍㩷㩿㫋㪈㩷㪄㩷㪫㪇㪀
㫋㪼㫊㩷㪄㩷㪫㪇
After correction
(Unknown sample)

NOTE
Time before base time T0 is not corrected.

4.5.7 Sensitivity Compensation of RI Detectors

RI detectors have a characteristic that the sensitivity lowers when the molecular weight is low. When the molecular
weight distribution of the measuring sample is broad, sensitivity change of the RI detector affect the result.
First, a sample that is a mixture of equal quantity of standard samples that have different molecular weights
is analyzed, and the area values for each molecular weight are calculated. The area to be standard is
pre-determined, and the relationship between each molecular weight and the area is defined as shown in
the following figure.
Standard area value

Area
b (Standard area)

M Molecular weight

Molecular weight calculation is performed using a value obtained by multiplying the response amount (the
slice value), a molecular weight M, by b/a.

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In the [GPC Data Comparison] window, the GPC molecular weight calculation results from multiple
data files can be displayed in layers as one graphed view, and the statistical calculations are
performed. GPC data comparison is performed per detector/channel. When a data file contains
multiple calculation results of molecular weight distribution, you can specify a detector/channel and
display the data in one graph.

5.1 Displaying the [GPC Data Comparison] Window

1 Click the

Postrun] program.
(GPC Data Comparison) icon in the [Main] assistant bar of the [GPC

The [GPC Data Comparison] window appears.

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5.2 Using and Viewing the [GPC Data Comparison] Window

The [GPC Data Comparison] window contains [Data List View], [Chromatogram View], [Molecular Weight
Distribution View], and [Cumulative Percent View].
[Data List View] [Molecular Weight Distribution View]

[Chromatogram View] [Cumulative Percent View]

No. Description
A data list for the GPC data comparison is displayed.
Chromatograms are displayed in layers.
Differential molecular weight distribution curves are displayed in layers.
Integral molecular weight distribution curves are displayed in layers.
Click this button (change size button) to switch between the full screen and standard displays.

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5.3 Data List View

A list of data for the GPC data comparison is displayed in [Data List View].

No. Description
Data files are numbered in the order registered.
Data file names are displayed.
Detector/channel names are displayed.
Names of the method files that were used to measure the data are displayed.
Sample names of data files are displayed.
5
5.4 Chromatogram View
Chromatograms of data registered in [Data List View] are displayed in layers in [Chromatogram View].

No. Description
Click to shift the chromatograms in the X axis direction using the mouse.
Displays the data list numbers, data file names, detector names, and channel names in the graph display colors.
The time (or amount of elution) and the intensity (%) at the mouse position when the mouse is located on the
graph.
(The time and the amount of elution can be switched by the axis configuration of the graph.)
Marker lines.

NOTE
Dragging on the chromatogram to specify the range will zoom in on that range.

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5.4.1 Display of Marker Lines

Two marker lines are displayed on the left and right of the chromatogram. They can be moved along the X
axis by dragging them. This forces the marker lines of the differential molecular weight distribution and the
integral molecular weight distribution curves automatically move to the corresponding new positions. Also,
by dragging the marker line on the differential or integral molecular weight distribution graphs you can view
the corresponding position on the chromatogram.

NOTE
When the chromatograms are overlayed using different calibration curves, marker lines are displayed but
they are not functional.

5.4.2 Shifting Chromatograms

There are two methods to shift chromatograms in the X axis direction: setting the shift time in the [Shift
Chromatogram Data] screen and using the (Move Left/Right) button.
The corresponding differential molecular weight distribution curve graph and integral molecular weight
distribution curve graph are shifted accordingly.

 Using the [Shift Chromatogram Data] Screen

1 Right-click in the chromatogram view and click [Move Left/Right] from the menu.

2 Configure the settings.

No. Description
Select a data file name to shift.
Numbers and file names registered in the data list are displayed in the combo box.
Displays the shift time from the initial state. You can also directly enter the shift time.
The X axis scale indicates the total shift time (min) or the total shift elution amount (mL).
The total shift time is increased or decreased from the current position only by the unit specified in
[Shift Time].
The unit used for increasing and decreasing in can be selected from among [0.00001], [0.0001],
[0.001], [0.01], [0.1] and [1.0].

3 Click [OK] or [Apply].

The chromatogram is shifted according to the time specified in the total shift time.

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 Using the Move Left/Right Button

1 Click the (Move Left/Right).

It becomes as if the button is held down.

2 Drag from the start position to the end position on the chromatogram.
Placing the cursor on the chromatogram will display a vertical line. Drag to specify a range.
The selected data of the chromatogram will be shifted, and the corresponding graph of the differential
molecular weight distribution curve and the graph of the integral molecular weight distribution curve will
also be shifted.

5.4.3 Display Settings of Chromatograms

Display settings for [Chromatogram View] are performed as follows:

1 Right-click on the chromatogram and click [Display Settings] from the menu.

2 Configure the settings. 5

No. Description
Select the display method of the Y axis scale of the chromatogram.

3 Click [OK].

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5.4.4 Setting View Properties

1 Right-click on the chromatogram and click [Properties] from the menu.

2 Click the [General] tab and configure the settings.

No. Description
Displays or hides the X axis grid.
Displays or hides the Y axis grid.
Displays or hides the graph comments.
When [Display Y-axis value at intensity.] is checked, the Y-axis coordinate of the mouse will be
displayed in the intensity value.
Displays or hides the magnification buttons (zoom-in and zoom-out).
Displays or hides the Y axis scroll bar.
Displays or hides the detector and channel names.

3 Click the [Color] tab and configure the settings.

No. Description
Allows you to select a display item, such as [Graph Background] or [Graph Frame], for the color setting.
Displays the color set for the selected display item.
Sets the color of the selected display item.
Click this to set the color of the display item.

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 5.4 Chromatogram View

4 Click the [Font] tab and configure the settings.

No. Description
Allows you to select an item for the font setting.
Displays the selected [Font Name], [Font Size], and [Font Style].
Displays the [Font] screen. Select a font.

5 Click [OK].
5

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5.5 Molecular Weight Distribution View

In [Molecular Weight Distribution View], differential molecular weight distribution curves of data registered
in the data file list are displayed in layers.

No. Description
Displays the data list numbers, data file names, detector names, and channel names in the graph display colors.
Displays the molecular weight (or degree of polymerization) and its percentage at the mouse position if the
mouse is located on the graph.
These are displayed in conjunction with the marker lines of the chromatogram.

NOTE
You can zoom in on an area on the differential molecular weight distribution curve graph by dragging a
square to specify the area.

5.5.1 Display of Marker Lines

Two marker lines are displayed on the differential molecular weight distribution curve graph. They can be
moved along the X axis by dragging them. This forces the marker lines of the chromatogram and the
integral molecular weight distribution graph move automatically with them.

5.5.2 Display Settings of the Differential Molecular Weight Distribution Curve

Reference
"5.4.3 Display Settings of Chromatograms" on page 81

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 5.5 Molecular Weight Distribution View

5.5.3 Setting View Properties

1 Right-click on the differential molecular weight distribution curve graph, and click
[Properties] from the menu.

2 Click the [General] tab and configure the settings.

No. Description
Displays or hides the graph comments. Select either [M.W./D.P] or [log (M.W.) /log (D.P.)] for the
scale display when displaying the molecular weight information (molecular weight or degree of
polymerization) in a graph comment. When [Display Y-axis value at intensity.] is checked, the Y-axis
coordinate of the mouse will be displayed in the intensity value.

3 Click the [Color] tab and configure the settings.

Reference
For details, see step 3 (P.82) in "5.4.4 Setting View Properties".

4 Click the [Font] tab and configure the settings.

Reference
For details, see step 4 (P.82) in "5.4.4 Setting View Properties".

5 Click [OK].

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5.6 Cumulative Percent View

In [Cumulative Percent View], integral molecular weight distribution curves of data registered in the data file
list are displayed in layers.

No. Description
Displays the data list numbers, data file names, detector names, and channel names in the graph display colors.
Displays the molecular weight (or degree of polymerization) and its percentage at the mouse position if the
mouse is located on the graph.
These are displayed in conjunction with the marker lines of the chromatogram.

NOTE
You can zoom in on an area on the integral molecular weight distribution curve graph by dragging a square
to specify the area.

5.6.1 Display of Marker Lines

Two marker lines are displayed on the integral molecular weight distribution curve graph. They can be
moved along the X axis by dragging them with a mouse while the marker lines of the chromatogram
and the differential molecular weight distribution curve graph are moved as well.

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 5.6 Cumulative Percent View

5.6.2 Setting View Properties

1 Right-click on the integral molecular weight distribution curve graph, and click
[Properties] from the menu.

2 Click the [General] tab and configure the settings.

No. Description
Displays or hides the graph comments.
Select either [M.W./D.P] or [log (M.W.) /log (D.P.)] for the scale display when displaying the molecular
weight information (molecular weight or degree of polymerization) in a graph comment.
When [Display Y-axis value at intensity.] is checked, the Y-axis coordinate of the mouse will be
displayed in the intensity value.

3 Click the [Color] tab and configure the settings.

Reference
For details, see step 3 (P.82) in "5.4.4 Setting View Properties".

4 Click the [Font] tab and configure the settings.

Reference
For details, see step 4 (P.82) in "5.4.4 Setting View Properties".

5 Click [OK].

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5.7 Statistical Calculation Result

1 Click [Statistical Calculation Results] from the [View] menu.

2 Configure each setting.

No. Description
Select either [Total] or [Optional Area] for the display of the statistical results.
Select the No. to display when [Optional Area] is selected in the type.
Displays the data list and statistical calculation results. The display items can be specified in [Table
Style]. The maximum number of data for the GPC data comparison is 10.

3 Click [OK].

NOTE
The display items are set in the [Table Style] screen. When all data cannot be displayed in the data list grid
display area, the vertical and horizontal scroll bars are used to scroll the table.

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 5.7 Statistical Calculation Result

5.7.1 Setting the Display Items of the [Statistical Calculation Result] Screen

1 Right-click on the data table in the [Statistical Calculation Results] screen, and click
[Table Style] from the menu.

2 Click the [Item Settings] tab and configure the settings.

5
No. Description
Moves items selected in [Hide Items] to [Display Items].
Moves items selected in [Display Items] to [Hide Items].
Click these to move a selected item in [Display Items] up or down.

3 Click the [Font] tab and configure the settings.

No. Description
Enter or select a font name.
Enter or select a font style.
Enter or select a font size.
Select a text color.
Character display sample based on the setting is displayed.

4 Click [OK].

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6 Report Function

This chapter describes the report function of LabSolutions GPC software.

In addition to the LC Real Time Analysis print items, the following GPC analysis print items are available.

• GPC calibration curve item

Allows you to print a calibration curve, a calibration curve table, and calibration curve correction parameters.
• GPC graph item
Allows overlay printing of a chromatograph and a calibration curve, as well as a differential and an integral
curves, from a data file.
• GPC overlay item
Allows overlay printing of the same type of curves from multiple data.
• GPC calculation result item
Allows printing of slice information, peak information, and average molecular weight information.
• GPC summary item (molecular weight statistic values of multiple data)
Allows you to print molecular weight statistic values from multiple data.
• Method (GPC parameters)
Allows printing of GPC method parameters.

Click any of the following icons to select the items.

6

No. Icon Item

GPC calibration curve

GPC graph

GPC overlay

GPC calculation result

GPC summary

Method

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6.1 Creating Reports

Create a GPC analysis result report as follows:

1 Click (Report Format) in the [Main] assistant bar of the [GPC Postrun] program.

2 Click the print item you would like to paste.

For example, click (GPC Calibration Curve).

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 6.1 Creating Reports

3 Select the area where you wish to paste the print item.
On the report creation screen, drag the cursor from the starting point to the ending point of the area where
you wish to paste the print item (the calibration curve).

4 Specify the print item format.

Set the print position, the size, the font, etc. on the [General] tab. 6

No. Description
Specify the distance from the upper left corner of the report paper in mm with the [Left] and [Top] print
positions.
Specify the size (in mm) of the print item in [Width] and [Height].
Displays the font used for printing.
Check [Enable] to display the report with a title.
Select an item to set a color for.

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5 Click the [File] tab, and specify a file for the print item.

No. Description
Specify a file name to print a report.
Check this box to fix the file to print.
Click this to display the file selection screen.

6 Click [OK].
The print item is displayed on the report creation screen.

If you wish to change the position or size of the print item displayed, display the property again and make
necessary changes.

Click (Print) on the [Report] assistant bar to print the created report.

NOTE
When you drag and drop a data file from [Data Explorer] to the report creation screen, the actual data
is imported and displayed on the report creation screen.

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 6.2 GPC Calibration Curve Item

6.2 GPC Calibration Curve Item

The calibration curve information used for GPC analysis is laid out on the report as follows:
The header, the calibration curve graph, and the calibration curve table can be configured separately.

No. Description
Header (Calibration curve correction parameters and calibration curve equation information are
displayed.)
Calibration curve graph
Calibration curve table
6

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6 Report Function

 Setting Position
Positions of the graph and the table in the GPC calibration curve item are set.

1 Right-click on an item pasted on the report, and click [Properties] from the menu.
Double-clicking the item also displays the screen.

2 Click the [Position] tab and configure the settings.

No. Description
Select a detector and a channel to be printed from the combo box.
Check [Fix Detector Ch No.] to fix the detector number.
Check this box to display the header and calibration curve correction parameters.
Check this box to print the calibration graph. Set the width of the graph optionally. If you make the
graph width bigger, the table width becomes smaller.
Check this box to display the calibration table.
Check this box to set the position and size of the graph and the table individually.

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 6.2 GPC Calibration Curve Item

 Setting the Header

Set what is to be printed in the header.

1 Click the [Header] tab and configure the settings.

6
No. Description
Enter and edit letters to be printed.
Click to restore the text edit area to the initial values.
Select how you want to align the text in the item frame from the list.
Specify the number of spaces for a tab stop. Click [] or [] on the right side or directly enter a
desired numeric value.
Variables can be inserted into the header information. Select them from the variable list. After
selecting, click [Insert] to insert the variable at the cursor position in the text edit area.

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 Setting a Graph
Specify how to print out the calibration graph as follows:

1 Click the [Graph] tab and configure the settings.

No. Description
Specify the axis types and axis names to be printed on the graph. Check [X] or [Y] to display the
graph scale of the axis. $CalibXAxis$ and $CalibYAxis$ can be used as axis names.
Check these boxes to print a title for each position.
Check this box to display grid lines.
Check this box to display a calibration point number on each calibration point.
Select the mark type of the calibration points.
Check this box to fill the marks when [Circle] or [Square] is selected in [Mark Type].
Select the horizontal axis of the graph.
Select the method for specifying the display range of the calibration curve. Choosing [User Defined]
allows you to set the display range.

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 6.2 GPC Calibration Curve Item

 Setting the Table

Set the print format of the calibration table as follows:

1 Click the [Table] tab and configure the settings.

6
No. Description
Move the item selected in [Hide Items] to [Display Items].
Move the item selected in [Display Items] to [Hide Items].
Move the item selected in [Display Items] up or down.
Select a column name to be displayed in the column index row.
Select the display position for an item from the list.
Set the number of characters that can be referred to.
Set the width of the column.
Click this to display the [Format Settings] screen. (See below)
Check to display the column index.
Set the cell height.

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Setting the format

Set the data format to display in the item frame related to table as follows:
In the properties of the item frame for printing information in a table format, such as in a peak table, click
[Format] in the [Format] tab or [Table] tab to display the [Format] screen.

NOTE
[Format] is enabled when one item of the [Display Items] is selected.

No. Description
Enter characters to be added in the front of data.
Enter characters to be added in the back of data, such as unit.
Set the format related to values.
Check the box to enable the number-of-decimal-digits setting, and enter the number in the text box.
Select the way to round numbers.
Check this box to enable the minimum data length setting. Then enter the minimum data length for
the data.
Set the placement within the display range for when the value is less than the minimum data length.
From the list displayed by clicking [] on the right side, select characters to fill in blank digits for the
data shorter than the minimum length.
Set characters to replace the value if it is 0 (zero).

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 6.3 GPC Graph Item

6.3 GPC Graph Item

This item is used to create the print layout for graphs used for GPC analysis.
• Chromatogram
• Calibration curve
• Differential molecular weight curve
• Integral molecular weight curve
These curves in a data file can be output in two graphs.
Combinations which you can overlay are as follows.
• Chromatogram and calibration curve
• Differential curve and integral curve (The horizontal axis is the molecular weight.)

 Setting Graphs to Be Output

Set graphs to be output on report as follows:

1 Right-click on an item pasted on the report and click [Properties] from the menu.
Double-clicking the item also displays the screen.

2 Click the [Graph] tab, and configure the settings.

6

No. Description
Select a detector and a channel of chromatogram to be output.
Check [Fix Detector Ch No.] to fix the detector number.
Check this box to display the chromatogram.
Check this box to display the calibration curve.
Check this box to display the differential curve.
Check this box to display the integral curve.
Select the display unit (min. or mL) of the horizontal axis of the graph.
Check to display annotations. Click [Details] to set the content to be displayed as annotations.
Check these boxes to display the baseline or peak detection marks on the chromatogram.
Check [RT/MW Range Mark] to display marks for the RT/MW range segment.
Check to display the average molecular weight on the graph. The calculation method for the average
molecular weight can be selected from either the detection peak unit or from the entire chromatogram.
Check [Draw Average Molecular Weight Mark] to draw a mark noting the average molecular weight.

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6.4 GPC Overlay Item

This item is used to overlay graphs of the channel to which the GPC calculation is specified to be done from
multiple data files. This is used when you wish to compare chromatograms or differential curves.

NOTE
• Different types of curves, such as differential curves and integral curves, cannot be overlayed on a
graph.
• Reports with GPC overlay items can be output from the [Report Generator] window.

 Setting Graphs to Be Output

Select the type of curves to be overlayed, and specify the output channels.

1 Right-click on an item pasted on the report and click [Properties] from the menu.
Double-clicking the item also displays the screen.

2 Click the [Graph] tab in the [GPC Overlay Properties] screen, and configure the settings.

No. Description
Specify channels to print report.
Check [Fix Detector Ch No.] to fix the detector number.
Select the way to set x-axis display range, automatically or manually.
Specify the graph output range in time, molecular weight, or log(molecular weight).
Check this box to display grid lines in the graph.
Select one from chromatogram, molecular weight distribution curve, and cumulative percent curve.
Check to display annotations. Click [Details] to set the content to be displayed as annotations.
Select the horizontal axis of the graph.
Select the way to display the graph. This selection is disabled for a cumulative percent curve.

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 6.5 GPC Summary Item

6.5 GPC Summary Item

This item is used to perform statistical calculations with molecular weight calculation results from multiple
data and to calculate and output the statistics (average, %RSD, maximum value, minimum value, and
standard deviation) to report.
You can select whether to perform statistical calculations by chromatogram or RT/MW range.
Statistical calculations are performed for the following molecular weight calculation results.
• Number average molecular weight (Mn)
• Weight average molecular weight (Mw)
• Z average molecular weight (Mz)
• Z+1 average molecular weight (Mz1)
• Viscosity average molecular weight (Mv)
• Mw/Mn
• Mv/Mn
• Mz/Mw
• Intrinsic viscosity

Printing by chromatogram
Statistical calculation is performed with the total molecular weight of the channel in which "GPC
Calculation" is enabled at the selected channel, and statistic values are output in the following print image.

6
Adding data files
increases number of
these rows. You can configure which
statistical calculation values
should be included in the table.

Printing by RT/MW range

Various types of average molecular weight are displayed by RT/MW range of the channel in which GPC
calculation is enabled, and the statistic values are output. Since you can set multiple RT/MW ranges for a
channel, statistical calculations are performed in the ascending order of the retention time of each channel.
• RT/MW range mapping image

The time range of RT/MW range set by data differs, but statistical calculations are performed by RT/MW
range where the retention time is short, and the results are output.
Output image

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6 Report Function

 Setting Summary Output

Select the statistical values for the output and whether the output is by chromatogram or by RT/MW range.

1 Right-click on an item pasted on the report and click [Properties] from the menu.
Double-clicking the item will also display the [Properties].

2 Click the [Output] tab, and configure the settings.

No. Description
Specify the channel to be output. Check [Fix Detector Ch No.] to fix the detector number.
Check this box to display the chromatogram.
Set the chromatogram to be output by [Each Data], [Each Detector], or [Each Channel].
Set the lateral arrangements of the chromatogram. You can also set the width between the blocks.
Check to display the summary table. Specify the output unit as either [Each Chromatogram] or [Each
RT/MW]. You can also specify the display ratio of the chromatogram and the table.
Check this box to display data file names in the title column with their paths.
Check [Baseline] to connect peak baselines with a line.
Check [Peak Detection Mark] to display peak detection marks.

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 6.5 GPC Summary Item

 Setting Summary Table (Column Setting)

Set column configuration of the summary calculation result table as follows:

1 Click the [Summary Table] tab, and configure the settings.

6
No. Description
Move the item selected in [Hide Items] to [Display Items].
Move the item selected in [Display Items] to [Hide Items].
Move the item selected in [Display Items] up or down.
Select a column name to be displayed in the column index row.
Select the display position for an item from the list.
Set the number of characters that can be referred to.
Set the width of the column.
Click to display the [Format] screen.
Check this box to display the column index.
Set the cell height.

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6 Report Function

 Setting Summary Table (Setting Title)

Set row configuration of the summary calculation result table as follows:

1 Click the [Summary Table Title] tab, and configure the settings.

No. Description
Move the item selected in [Hide Items] to [Display Items].
Move the item selected in [Display Items] to [Hide Items].
Move the item selected in [Display Items] up or down.
Set the title to be displayed in the row index column (leftmost column).
Select the display position for an item from the list. (Position setting is available only for %RSD.)
Click to show the [Format] screen. (Format setting is available only for %RSD.)

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 6.5 GPC Summary Item

 Setting Files
Set the list of data files to create a summary.

1 Click the [File] tab, and configure the settings.

6
No. Description
Click to display the [Open File] screen. Select the data files.
Displays a data file list.
Check this box for a fixed file name to print.
Click this to remove the file selected in the data file list from the calculation target, and delete it from
the list.
Click the [Up] or [Down] to change the order of files in the data file list.

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6 Report Function

6.6 Method Item

Method parameters of the GPC analysis can be output from the conventional method report items.
Following parameters that are necessary for GPC analysis are output.
• Title
• Element name
• Q factor
• Alpha value
• Κ value
• Flow setting
• Flow value
• Molecular weight distribution
• Molecular weight per degree of polymerization
• Channel number
• Filtration limit time
• Time correction method
• Time correction parameters
• Delay time correction
• Sensitivity Compensation of RI Detectors
• RT/MW range setting

 GPC Parameters

1 Right-click on an item pasted on the report and click [Properties] from the menu.

2 Click the [Data Processing Parameters] tab and configure the settings.

NOTE
The GPC items are [GPC Parameters] and [GPC Method].

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 6.6 Method Item

 Setting [Details of GPC Parameters]

Set the output items for method information set as GPC parameters.

1 Select [GPC Parameters] from the [Display Items], and click [Detail].

2 Configure the settings.

NOTE
All items are [Display Items] by default.
Move items that should not be output to [Hide Items] and click [OK].

 Setting [Details of GPC Methods]

Set the output items for method information for each channel set as the GPC method.

Reference
See " Setting [Details of GPC Parameters]" for operation of the [Detail] screen of the GPC methods.

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6 Report Function

6.7 GPC Calculation Result

Slice information, peak information, and various average molecular weight information are output as the
GPC calculation result. The calculation result can be output by peak or RT/MW range.

• [Block] is set at [Style]

• [Table] is set at [Style]

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 6.7 GPC Calculation Result

 Output Setting
Select whether the GPC calculation result is output by peak or by RT/MW range. You can also select
calculation result items of slice information, peak information or average molecular weight.

1 Right-click on an item pasted on the report and click [Properties] from the menu.

2 Click the [Output] tab, and configure the settings.

No. Description
Select whether the calculation result is printed by peak or by RT/MW range.
Select the style for outputting the calculation result.
Select items to display as calculation result. Uncheck all to display only titles such as peak number
or RT/MW range number.

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 Slice Information
Set the display format of the slice information as follows: You can set the display format by grid.

1 Click the [Slice Data Information] tab, and configure the settings.
The [Slice Data Information] screen appears.

No. Description
Move the item selected in [Hide Items] to [Display Items].
Move the item selected in [Display Items] to [Hide Items].
Move the item selected in [Display Items] up or down.
Select a column name to be displayed in the column index row.
Select the display position for an item from the list.
Set the width of the column.
Click to display the [Format] screen.
Check this box to display the column index.
Set the cell height.
You can select whether to display all of the slice information or to skip a certain number of slices.

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 6.7 GPC Calculation Result

 Peak Information
Set each title of the peak information data.

1 Click the [Peak Information/Range Information] tab, and configure the results.

6
No. Description
You can select title items of the peak information and change the titles.
Start: Peak start time, Top: Peak top time, End: Peak end time

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6 Report Function

 Average Molecular Weight

Set the print output format of the average molecular weight result.

1 Click the [Average Molecular Weight] tab, and configure the settings.

No. Description
You can select fonts of the molecular weight result to be output. You can emphasize the molecular
weight result by selecting fonts.

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7 GPC File Converter

The GPC file converter provides the following functions:

• Importing of method files created by CLASS-LC10/CLASS-VP GPC software
• Creation of files by merging a LabSolutions method file and a CLASS-LC10/CLASS-VP GPC method file
The conversion items in a file are as follows:
• User who created the file, date created, comment
• GPC parameters (such as GPC parameters, correction parameters, RT/MW range table)
• Calibration curve information

7.1 Importing a Method File

Import the information of a CLASS-LC10/CLASS-VP GPC method file by opening it.
Information of a CLASS-LC10/CLASS-VP method file is imported to the current detector channel.

1 Click [Import CLASS GPC Method File] from the [File] menu in the [GPC Calibration
Curve] window.

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7 GPC File Converter

2 Select a CLASS GPC method file and click [Open].

A CLASS-LC10/CLASS-VP GPC method file is opened.

7.2 Merging GPC Method Files

Create a new LabSolutions method file by merging an existing LabSolutions method file and a
CLASS-LC10/CLASS-VP GPC method file.
Information of a CLASS GPC method file is deployed to all the channels of detectors in a LabSolutions method file.

1 Click [Merge of CLASS GPC Method File] from the [File] menu of the [GPC Calibration
Curve] window.

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 7.2 Merging GPC Method Files

2 Specify file to be merged and click [Merge].

No. Description
Select or input [Method file(LabSolutions)].
Select or input [CLASS GPC method file].
Select or input [Output method file name(LabSolutions)].

NOTE

• When you select or input the name of an existing file in , the file is overwritten.
• If there are multiple files you wish to merge, repeat the above.

3 Click [Close].
If the audit trail setting is enabled with a method file, a CLASS-LC10/CLASS-VP GPC method file cannot
be merged with it.

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8 Appendix

This chapter details help for problems with software operation, the method for checking the content of
online manuals, and information on LabSolutions GPC specifications.

8.1 When Having Problems with the Operation

This software provides the help menu and online manuals. Use these for any uncertainties in operation and
the terms on the screen.

8.1.1 Using Help

To check the help for this software, open the help using one of the following methods.

Operation Type Operation Method

[Help] Click [Help] on the screen.
Display the help for the open window.
(Contents) Click (Contents) in the toolbar.
[Help] menu Click [Contents] from the [Help] menu.
[F1] key Press the [F1] key on the keyboard.
To verify the content of the open screen, press the [F1] key to display the help that corresponds
to the open screen.

 Searching by Keyword
For unfamiliar terms and parameters, enter a keyword and search. A list of topics matching the keyword
8
will be displayed. You can view the help that matches the term or parameter from the topic.

1 Open the help.

2 Click the [Index] tab, and perform a search.

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8 Appendix

1 Enter a keyword to search, and press the [Enter] key on the keyboard.
Topics that match the keyword will be displayed in alphabetical order.
2 Click a topic.
3 Click [Display].
The content of the selected topic will be displayed.

NOTE
• When there are multiple topics for a specific keyword, the [Topics Found] screen is opened. Select
the specific keyword from the list of titles on the screen, and click [Display].

• The [Search] tab of the help allows full-text searching of help content for terms registered in the
help.

8.1.2 Using Online Manuals

The contents of the 'Operation Manual' and 'Administration Manual' of the LabSolutions Instruction Manual
can be viewed on the screen.

1 Click (Manual) on the [LabSolutions Main] window.

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 8.2 Specifications of LabSolutions GPC

2 Click the icon of the instruction manual to be viewed.

"Adobe Reader" is started, and the instruction manual is opened.

No. Description
Click the hierarchically structured bookmarks (table of contents) to display the desired page.
8
Search for terms used in the manual.
Click the references or the terms in blue to jump to the page of the related item.

NOTE
• The "Operation Manual" online manual can be opened by clicking [Online Manual] from the [Help]
menu.
• Adobe Reader is started for opening the online manual.
• Visit the Adobe web site for more information about Adobe Reader.

8.2 Specifications of LabSolutions GPC

 Control Device
• Up to 4 systems can be controlled by one PC, and PDA detectors can be connected to 2 of these systems at
maximum.
• GPC analysis and ordinary analysis can be performed simultaneously on one PC.
• Data from a GPC or LC device from other manufacturers can be processed by installing an A/D board on the
system controller (CBM-20A, SCL-10Avp).

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8 Appendix

NOTE
Number of controllable systems and the type of controllable devices depend on the LabSolutions license.

 Peak Integration
• Peak integration by peak integration parameters, time programs and manipulations can be performed.
• Number of slices: Up to 5000 (by channel. Up to 4 channels can be used in a PDA detector.

 Data Analysis
Calibration Type Normal, Broad(Area%), Broad(Hamielec)
Calibration curve Straight line by the least square method, third order equation, third order equation +
approximate equations hyperbolic curve, fifth order equation, fifth order equation + hyperbolic curve, seventh order
equation, seventh order equation + hyperbolic curve, and point-to-point
Number of data in Up to 64
calibration curve
Calibration curve Conversion using the Q factor or Mark-Houwink equation.
correction
Time correction Time correction using the internal standard peak or the control sample.
GPC calculation Number average molecular weight (Mn), weight average molecular weight (Mw), Z average
molecular weight (Mz), Z+1 average molecular weight (Mz1), viscosity average molecular
weight (Mv), intrinsic viscosity, and polydispersity (such as Mw/Mn) calculations for the
entire elution curve, for each peak, and for each specified range.
Others Correction of the RID sensitivity.

 Data Comparison
• Number of data files that can be compared: Up to 10
• Chromatograms, the differential molecular weight distribution curve, and the integral molecular weight
distribution curve can be overlayed.
• Parallel displacement of chromatograms in the direction of time axis, recalculation of the molecular weight
distribution by the correction time after parallel displacement, and displaying the molecular weight
distribution curve are possible.
• Statistical calculation results (average, maximum, minimum, %RSD, standard deviation) of the number
average molecular weight (Mn), the weight average molecular weight (Mw), the Z average molecular weight
(Mz), the Z+1 average molecular weight (Mz1), the viscosity average molecular weight (Mv), the intrinsic
viscosity, and the polydispersity (such as Mw/Mn) for the entire elution curve or any specified range can be
displayed.

 Report
• Reports can be created using flexible formats by selecting the report items (such as chromatogram,
calibration curve, GPC graph, GPC overlay, and GPC calculation result).
• The output of summary reports and statistical calculation results (average, maximum, minimum, %RSD,
standard deviation) using the GPC summary items is possible.
• Multiple page layout is supported.
• Reports can be previewed.

 File Conversion
• GPC method files of CLASS-LC10/CLASS-VP GPC software can be imported.
• GPC calculation results can be output into an ASCII file.
• LCsolution GPC data files and method files can be read as they are. However, files created with
LabSolutions GPC cannot be read by LCsolution GPC.

122 LabSolutions GPC Instruction Manual