Mahnaz (Zahra) Borzooeian, PhD

The long-term goal of this research was to understand how neurotransmitter transporters regulate synaptic and presynaptic neurotransmitters concentrations normally and how ischemia disrupts their homeostasis to produce excitotoxicity. I collaborated in the second part of the project focusing on comparing the effect of injection of some neurotransmitter agonists through brain injection. I acquired deep knowledge of aseptic surgery, mouse craniotomy, stereotaxic surgery, and immunohistochemistry.

Immobilization on a solid carrier is a method to improve enzyme stability and reusability. The purpose of this research, which was my PhD’s dissertation, was an assessment of structure, function, and stability of conjugated lysozyme on singled wall carbon nanotubes. High chemical stability of conjugation was confirmed using analytical techniques such as X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, TEM and SEM microscopy and agarose gel electrophoresis. Post… Show more

Immobilization on a solid carrier is a method to improve enzyme stability and reusability. The purpose of this research, which was my PhD’s dissertation, was an assessment of structure, function, and stability of conjugated lysozyme on singled wall carbon nanotubes. High chemical stability of conjugation was confirmed using analytical techniques such as X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, TEM and SEM microscopy and agarose gel electrophoresis. Post conjugation changes of the enzyme were investigated using fluorescence measurements and evolution of kinetic parameters. The outcome of this research offers a new perspective on using bio-molecules attachment on nanomaterials in nano-scaffold and nano-devices designing for cell and drug delivery.
The results of this study were published in several journals such as “Journal of experimental nanoscience, Vol. 5, No. 6”, which cited by the SAO/NASA Astrophysics Data System: This site is hosted by the: High Energy Astrophysics Division at the, Harvard-Smithsonian Center for Astrophysics: http://adsabs.harvard.edu/abs/2010JENan...5..536B
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In this project, which was my master’s degree dissertation, I made effort to better understand the stability of thermophilic glucoamylase for turning a mesophilic glucoamylase into its thermophilic counterpart. Thermophilic enzymes with wider availability and lower cost have more application in industry. This enzyme, as a thermozymes, showed unique characteristics such as temperature, chemical, and pH stability. Our results showed that thermophilic glucoamylase, which can play an important role… Show more

In this project, which was my master’s degree dissertation, I made effort to better understand the stability of thermophilic glucoamylase for turning a mesophilic glucoamylase into its thermophilic counterpart. Thermophilic enzymes with wider availability and lower cost have more application in industry. This enzyme, as a thermozymes, showed unique characteristics such as temperature, chemical, and pH stability. Our results showed that thermophilic glucoamylase, which can play an important role in food, chemical, pharmaceutical, paper, and waste-treatment industries, is a good source of novel catalyst that is of great industrial interest. Show less

Parametric separation of carbon nanotubes (CNTs), especially based on their length is a challenge for a number of nano-tech researchers. I developed a new methodology to measure the lengths of the resulted nanotubes which would be a valuable quality control tool for the manufacture of carbon nanotubes of specific lengths in bulk quantities.
This method is a unique, sensitive, inexpensive, rapid and simple separation method for high precision separation and high-resolution visualization… Show more

Parametric separation of carbon nanotubes (CNTs), especially based on their length is a challenge for a number of nano-tech researchers. I developed a new methodology to measure the lengths of the resulted nanotubes which would be a valuable quality control tool for the manufacture of carbon nanotubes of specific lengths in bulk quantities.
This method is a unique, sensitive, inexpensive, rapid and simple separation method for high precision separation and high-resolution visualization which obviates the need for centrifugation, additional chemical analyses, and expensive spectroscopic techniques such as Raman spectroscopy to visualize CNT bands and measure the lengths.
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