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Experiment 3. The Test For Protein: Substance Reaction With Copper Sulphate and Sodium Hydroxide

This document describes an experiment to test for the presence of proteins using the Biuret test. Four test tubes were prepared containing 1% starch solution, 10% glucose solution, 1% albumen solution, and water. Sodium hydroxide and copper sulfate solutions were added to each test tube. Only the albumen solution produced a purple color, indicating a positive result for proteins. The water was used as a control. The Biuret test reaction produces a purple color in the presence of proteins due to a compound called biuret.

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Andrew Poole
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45 views4 pages

Experiment 3. The Test For Protein: Substance Reaction With Copper Sulphate and Sodium Hydroxide

This document describes an experiment to test for the presence of proteins using the Biuret test. Four test tubes were prepared containing 1% starch solution, 10% glucose solution, 1% albumen solution, and water. Sodium hydroxide and copper sulfate solutions were added to each test tube. Only the albumen solution produced a purple color, indicating a positive result for proteins. The water was used as a control. The Biuret test reaction produces a purple color in the presence of proteins due to a compound called biuret.

Uploaded by

Andrew Poole
Copyright
© © All Rights Reserved
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Download as DOC, PDF, TXT or read online on Scribd
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Experiment 3.

The test for protein


(a) Label four test-tubes 1-4.
1% starch solution into tube 1
10% glucose solution into tube 2
(b) Put about 20 mm (depth) of
1% albumen solution into tube 3
water into tube 4
(c) Pour into each tube, about mm !ilute so!ium h"!ro#i!e. ($%&' ()
(!) %!! to this about mm !ilute co))er sul)hate solution. *ha+e the tube si!ewa"s to
mi# the contents.
(e) &eturn the tubes to the rac+, lea,e for a few secon!s an! recor! the resulting colours
in "our noteboo+.
Substance Reaction with copper sulphate and sodium hydroxide
1
2
3
4
( $%&'. *o!ium h"!ro#i!e is caustic an! !issol,es clothing, s+in an! bench to)s. -t is
!estructi,e rather than !angerous so if an" is s)ilt on the bench, neutrali.e it at once
with an e/ual ,olume of !ilute h"!rochloric aci! an! wi)e !r". -f s)ilt on clothing !o the
same but follow with a wash in as much water as )ossible. -f s)ilt on the s+in !o not a!!
aci! but wash un!er the ta) until the 0soa)"0 feeling is remo,e!.
Protein test ).1
Experiment 3. Discussion
I 1he substances selecte! for testing are e#am)les of three of the )rinci)al t")es of
chemical substances in cells2 starch, sugar (glucose), )rotein (albumen).
3ith which of these sam)les !i! the reaction gi,e a )ur)le colour 4
2 1he substance which ga,e a )ur)le colour was a single e#am)le of its class of
substances. 3oul! "ou e#)ect all other sam)les of this class to gi,e the same reaction4
3 3hat was the )oint of using test-tube 4 with the water4
561' 1his test is calle! the 7biuret8 test. 79iuret8 is the name of the com)oun! which
gi,es the )ur)le colour.
Protein test ).2
Experiment 3. Discussion - answers
I 6nl" the albumen shoul! gi,e a )ur)le colour.
2 1he stu!ents shoul! not be )re)are! to ma+e a generali.ation about all )roteins from
a single reaction with albumen solution. -f albumen is a t")ical )rotein, then it is )roteins
that will normall" gi,e the biuret reaction. -n fact it is gi,en b" all substances ha,ing at
least two )e)ti!e lin+ages (-$6-5:-) which inclu!es all )roteins an! most )e)ti!es.
3 -t coul! be argue! that so!ium h"!ro#i!e an! co))er sul)hate will )ro!uce the
characteristic )ur)le colour whether or not )roteins are )resent but the reaction is
su))resse! in the )resence of glucose or starch. 1he control with test-tube 4 ma+es this
e#)lanation less li+el".
Protein test ).3
Experiment 3. The test for proteins - preparation
Outline 1he 9iuret test is a))lie! to starch, glucose an! albumen solutions to show that
onl" the )rotein gi,es a )ositi,e result.
Prior knowledge 5one, e#ce)t )erha)s that so!ium h"!ro#i!e has caustic )ro)erties.
Ad!nce prep!r!tion !nd m!teri!ls"
1% starch solution
10% glucose solution cm
3
)er grou)
1% albumen solution
so!ium h"!ro#i!e 25 or 10%
10 cm
3
)er grou)
1% co))er sul)hate solution
(;ilute h"!rochloric aci! shoul! be a,ailable to neutrali.e an" caustic so!a s)ilt.)
App!r!tus-per group
test-tube rac+ an! 4 test-tubes
4 labels or s)irit mar+er
(-nstructions for )re)aring the reagents are gi,en on ).04
1'%$:'&* 561'* Protein test ).4

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