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Introduction

The document discusses gastritis, which is inflammation of the stomach lining. It can be caused by infection with H. pylori bacteria or other factors like injury or pain relievers. The document then focuses on H. pylori, its association with gastritis, and a study examining the use of culture to diagnose H. pylori infection and determine antibiotic susceptibility in Filipino patients.

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181 views

Introduction

The document discusses gastritis, which is inflammation of the stomach lining. It can be caused by infection with H. pylori bacteria or other factors like injury or pain relievers. The document then focuses on H. pylori, its association with gastritis, and a study examining the use of culture to diagnose H. pylori infection and determine antibiotic susceptibility in Filipino patients.

Uploaded by

api-3718174
Copyright
© Attribution Non-Commercial (BY-NC)
Available Formats
Download as DOC, PDF, TXT or read online on Scribd
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Introduction

Gastritis is a term used to describe a group of conditions characterized by


inflammation of the lining of your stomach. Commonly, the inflammation of
gastritis results from infection with the same bacterium that causes most stomach
ulcers. Yet other factors — including traumatic injury and regular use of certain
pain relievers — also can contribute to gastritis.
Gastritis may occur suddenly (acute gastritis), or it can occur slowly over time
(chronic gastritis). In spite of the many conditions associated with gastritis, the
signs and symptoms of the disease are very similar: a burning pain in your upper
abdomen and, occasionally, bloating, belching, nausea or vomiting.
In some cases, gastritis can lead to ulcers and an increased risk of stomach
cancer. For most people, however, gastritis isn't serious and improves quickly
with treatment.
Helicobacter pylori is a gram-negative bacterium that colonizes the gastric
mucosa of more than half of the world's population Since its isolation in 1982, the
association between H. pylori infection and the subsequent development of
chronic/acute active gastritis has been well established. The principal reservoir of
infection is the human stomach and transmission has been epidemiologically
linked to person to person contact . The prevalence of infection is greater in
developing countries and is influenced by socioeconomic conditions, ethnic
background and age In the Philippines, there is scarcity of published data
regarding the epidemiology of this bacterium. Locally unpublished reports
revealed a prevalence of 5.6% seropositivity rate in children and 60% among 136
adult Filipino patients with dyspepsia using the Clotest® (Cabahug et. al. 2003
and Caballero et al., 1997, unpublished data). A lower prevalence rate of 42%
was reported by Daez et. al. in 2002 (unpublished data) among 375 patients
undergoing endoscopy at the Philippine General Hospital utilizing the rapid
urease test and histopathology.
Microbiological isolation of the organism is the theoretical gold standard for the
detection of H. pylori infections. However, isolation of the organism by culture has
been highly variable. Success rates depend on the technical expertise of the
microbiology laboratory, ranging from 30% to 73%. Failure to detect the organism
may be due to sampling error, inappropriate transport or culture media and
insufficient incubation period.
In clinical practice, gastric biopsy with culture is not routinely performed due to
the availability of more rapid diagnostic tests in the detection of H. pylori such as
urease broth tests, urea breath tests, serologic methods and stool antigen
detection. However, the increasing prevalence of resistant strains makes culture
and antibiotic sensitivity testing valuable to determine alternative treatment
regimens after failure of initial eradication regimen.
In the Philippines, due to methodological difficulties in isolating the organism,
detection of the organism by culture methods has not been popular. Realizing the
increasing prevalence of antimicrobial resistance in other countries and its
potential negative impact on the efficacy of many treatment eradication regimens,
it is important in clinical practice to determine the prevailing local antibiotic
susceptibility patterns when choosing appropriate eradication regimens for H.
pylori infections in the empiric setting.
This study aims to evaluate the use of culture in the diagnosis of H. pylori
infection among patients with dyspepsia, to determine the sensitivity and
specificity of culture technique in the detection of H. pylori infection, and to
determine the antibiotic susceptibility patterns of H. pylori organisms isolated by
culture among Filipino patients.
52 dyspeptic adult patients undergoing endoscopy from the Outpatient
Gastroenterology clinic of the University of the Philippines-Philippine General
Hospital underwent multiple gastric biopsy and specimens were submitted for
gram stain, culture, antimicrobial sensitivity testing, rapid urease test and
histology. Antimicrobial susceptibility testing was done by Epsilometer testing
(Etest) method against metronidazole, clarithromycin, amoxicillin, and
tetracycline.

Sixty percent (60%) of the study population was positive for H. pylori infection
(mean age of 44 years ± 13), 70% were males. H. pylori culture showed a
sensitivity of 45% (95% CI [29.5-62.1]), specificity of 98% (95%CI [81.5-100%]),
positive likelihood ratio of 19.93 (95% CI [1.254-317.04]) and a negative
likelihood ratio of 0.56 (95% CI [0.406-0.772]). All H. pylori strains isolated were
sensitive to metronidazole, clarithromycin, amoxicillin and tetracycline.

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