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ORFs AA size should not include the terminal codon translation * #771
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@@ Coverage Diff @@
## master #771 +/- ##
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+ Coverage 72.11% 72.53% +0.42%
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Files 243 243
Lines 9262 9267 +5
Branches 3124 3124
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+ Hits 6679 6722 +43
+ Misses 2284 2250 -34
+ Partials 299 295 -4
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@XingGao-PKI thank you for this excellent PR. Could you please set up a simple cypress test for it? Thank you! |
Close PR and Reopen to trigger the Blocked Cypress tests |
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Nice work @XingGao-PKI ! Thank you!
Published a new version 16.3.35 for you @XingGao-PKI |
When a DNA/RNA translated to AA sequence in ORFs, a terminal codon translation * is always included, this is not a valid AA, we should reduce one AA from the AA size.
E.g: this sequence
atgactgaatacaagcctactgtcaggttggctacaagagacgacgttcctagagccgtgagaactctggctgcagccttcgccgactaccccgccacgagacacaccgttgacccagatcggcatattgagagagtgactgaactgcaggagctgtttcttacaagagttggcctcgacataggcaaggtgtgggtggcggacgacggcgccgccgtggccgtctggaccactcccgaatcagttgaggctggcgccgtattcgctgagatcggcccgagaatggctgagctcagcgggagtaggctcgcggcacagcagcaaatggagggactgctggcaccacacaggcccaaagaacccgcctggttcctggcaaccgtcggtgtatctcccgatcatcaggggaaaggtctgggctctgccgtagtgctccctggcgtggaggcagctgagagagcaggagtacctgccttcttggagacctccgctccaaggaatcttcccttctatgaacggttgggcttcaccgtgacagccgacgtggaagtccccgaaggcccccgcacttggtgcatgacgaggaagcctggagcgtga
is translate to AA sequence MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*
This AA string size is 200, but the actual valid AA size should be 199.